自身抗原La（SS-B）作为双链RNA解旋酶的特性分析。
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme.
作者信息
Hühn P, Pruijn G J, van Venrooij W J, Bachmann M
机构信息
Institut für Physiologische Chemie, Johannes-Gutenberg Universität, Duesbergweg 6, D-55099 Mainz, Germany.
出版信息
Nucleic Acids Res. 1997 Jan 15;25(2):410-6. doi: 10.1093/nar/25.2.410.
Abstract
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is homologous to the double-stranded RNA binding protein kinase PKR, are discussed.
摘要
在分析La（SS-B）自身抗原的催化活性时，检测到一种依赖ATP的双链RNA解旋活性。来自不同物种的天然和重组La蛋白均表现出这种活性，且该活性可被单特异性抗La抗体抑制。La蛋白能够解开具有两个3'端突出或一个3'端和一个5'端突出的双链RNA底物。具有两个5'端突出的双链RNA不会被解开，这表明解旋至少需要一个3'端突出。文中讨论了La蛋白中可能参与双链RNA解旋的序列元件，如一个进化保守的假定ATP结合基序以及一个与双链RNA结合蛋白激酶PKR同源的元件。
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