编码自身抗原SS - B/La的大鼠cDNA克隆的分离：种属特异性变异的检测
Isolation of rat cDNA clones coding for the autoantigen SS-B/La: detection of species-specific variations.
作者信息
Semsei I, Tröster H, Bartsch H, Schwemmle M, Igloi G L, Bachmann M
机构信息
Institut für Physiologische Chemie, Universität, Mainz, Germany.
出版信息
Gene. 1993 Apr 30;126(2):265-8. doi: 10.1016/0378-1119(93)90378-g.
Clones of cDNA coding for the autoantigen La (or SS-B) were isolated from a library made from rat liver. A comparison of the rat La cDNA (encoding from nt 38 to 1281 for rat La protein) with the sequences known for human and bovine La protein resulted in the identification of species-specific inserts. The inserts seem to be the result of multiplication of flanking sequences during evolution. In addition to these variations, we observed that rat La cDNAs exhibit non-canonical polyadenylation sites. Finally, a databank search resulted in the identification of a DNA sequence originally termed as TAG or TSG20X (GenBank accession No. X61893) which represents the C terminus of mouse La/SS-B protein.
从大鼠肝脏构建的文库中分离出编码自身抗原La（或SS-B）的cDNA克隆。将大鼠La cDNA（编码大鼠La蛋白的第38至1281个核苷酸）与已知的人和牛La蛋白序列进行比较，鉴定出物种特异性插入片段。这些插入片段似乎是进化过程中侧翼序列倍增的结果。除了这些变异外，我们还观察到大鼠La cDNA表现出非典型的聚腺苷酸化位点。最后，通过数据库搜索鉴定出一个最初称为TAG或TSG20X的DNA序列（GenBank登录号X61893），它代表小鼠La/SS-B蛋白的C末端。
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