裂殖酵母中主要复制起点的鉴定。
Identification of a predominant replication origin in fission yeast.
作者信息
Okuno Y, Okazaki T, Masukata H
机构信息
Department of Molecular Biology, School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 564-01, Japan.
出版信息
Nucleic Acids Res. 1997 Feb 1;25(3):530-7. doi: 10.1093/nar/25.3.530.
Abstract
We have identified five autonomously replicating sequences (ARSs) in a 100 kbp region of the Schizosaccharomyces pombe chromosome II. Analyses of replicative intermediates of the chromosome DNA by neutral/neutral two-dimensional gel electrophoresis demonstrated that at least three of these ARS loci operate as chromosomal replication origins. One of the loci,ori2004, was utilized in almost every cell cycle, while the others were used less frequently. The frequency of initiation from the respective chromosomal replication origin was found to be roughly proportional to the efficiency of autonomous replication of the corresponding ARS plasmid. Replication from ori2004 was initiated within a distinct region almost the same as that for replication of the ARS plasmid. These results showed that the ori2004 region of approximately 3 kbp contains all the cis elements essential for initiation of chromosome replication.
摘要
我们在粟酒裂殖酵母二号染色体的100 kbp区域内鉴定出了五个自主复制序列（ARSs）。通过中性/中性二维凝胶电泳对染色体DNA复制中间体进行分析表明，这些ARS位点中至少有三个作为染色体复制起点发挥作用。其中一个位点ori2004在几乎每个细胞周期中都被利用，而其他位点的使用频率较低。发现从各个染色体复制起点起始的频率大致与相应ARS质粒的自主复制效率成正比。ori2004的复制在一个与ARS质粒复制几乎相同的特定区域内起始。这些结果表明，大约3 kbp的ori2004区域包含了染色体复制起始所必需的所有顺式元件。
相似文献
1
Identification of a predominant replication origin in fission yeast.裂殖酵母中主要复制起点的鉴定。
Nucleic Acids Res. 1997 Feb 1;25(3):530-7. doi: 10.1093/nar/25.3.530.
2
Chromosomal context and replication properties of ARS plasmids in Schizosaccharomyces pombe.粟酒裂殖酵母中ARS质粒的染色体背景与复制特性
J Biosci. 2015 Dec;40(5):845-53. doi: 10.1007/s12038-015-9572-y.
3
Mapping autonomously replicating sequence elements in a 73-kb region of chromosome II of the fission yeast, Schizosaccharomyces pombe.绘制粟酒裂殖酵母（Schizosaccharomyces pombe）二号染色体73千碱基区域中的自主复制序列元件图谱。
J Genet. 2007 Aug;86(2):139-48. doi: 10.1007/s12041-007-0018-7.
4
Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe.三个自主复制序列（ARS）元件对裂殖酵母粟酒裂殖酵母中的ura4复制起始区域有贡献。
EMBO J. 1994 Aug 1;13(15):3638-47. doi: 10.1002/j.1460-2075.1994.tb06671.x.
5
A replication-time-controlling sequence element in Schizosaccharomyces pombe.粟酒裂殖酵母中的一个复制时间控制序列元件。
Chromosoma. 2017 Aug;126(4):465-471. doi: 10.1007/s00412-016-0606-5. Epub 2016 Jun 20.
6
Comparison of the two major ARS elements of the ura4 replication origin region with other ARS elements in the fission yeast, Schizosaccharomyces pombe.将裂殖酵母粟酒裂殖酵母中ura4复制起始区域的两个主要自主复制序列（ARS）元件与其他ARS元件进行比较。
Chromosoma. 1994 Oct;103(6):414-22. doi: 10.1007/BF00362286.
7
Influence of a replication enhancer on the hierarchy of origin efficiencies within a cluster of DNA replication origins.复制增强子对DNA复制起点簇内起点效率层级的影响。
J Mol Biol. 1999 May 21;288(5):867-82. doi: 10.1006/jmbi.1999.2728.
8
Physical mapping of origins of replication in the fission yeast Schizosaccharomyces pombe.裂殖酵母粟酒裂殖酵母中复制起点的物理图谱
Mol Biol Cell. 1994 Aug;5(8):839-49. doi: 10.1091/mbc.5.8.839.
9
High density of weak replication origins in a 75-kb region of chromosome 2 of fission yeast.裂殖酵母 2 号染色体 75kb 区域中弱复制起点的高密度。
Genes Cells. 2010 Jan;15(1):1-12. doi: 10.1111/j.1365-2443.2009.01363.x. Epub 2009 Dec 9.
10
Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin.成簇的腺嘌呤/胸腺嘧啶片段对裂殖酵母复制起点的功能至关重要。
Mol Cell Biol. 1999 Oct;19(10):6699-709. doi: 10.1128/MCB.19.10.6699.
引用本文的文献
1
A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin.一种复合DNA元件，作为异染色质表观遗传遗传所需的维持因子发挥作用。
Mol Cell. 2021 Oct 7;81(19):3979-3991.e4. doi: 10.1016/j.molcel.2021.07.017. Epub 2021 Aug 9.
2
Chromosomal context and replication properties of ARS plasmids in Schizosaccharomyces pombe.粟酒裂殖酵母中ARS质粒的染色体背景与复制特性
J Biosci. 2015 Dec;40(5):845-53. doi: 10.1007/s12038-015-9572-y.
3
Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.粟酒裂殖酵母中复制起点、基因和基因间区域的应激诱导双链体去稳定化（SIDD）特性分析。
BMC Res Notes. 2012 Nov 19;5:643. doi: 10.1186/1756-0500-5-643.
4
Chromatin architectures at fission yeast transcriptional promoters and replication origins.裂殖酵母转录启动子和复制原点处的染色质结构。
Nucleic Acids Res. 2012 Aug;40(15):7176-89. doi: 10.1093/nar/gks351. Epub 2012 May 9.
5
Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.多种途径可以绕过裂殖酵母 Hsk1 激酶在 DNA 复制起始中的必需作用。
J Cell Biol. 2011 Oct 31;195(3):387-401. doi: 10.1083/jcb.201107025. Epub 2011 Oct 24.
6
Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.Mrc1 标记早期触发起源，并协调有丝分裂酵母中起始的时间和效率。
Mol Cell Biol. 2011 Jun;31(12):2380-91. doi: 10.1128/MCB.01239-10. Epub 2011 Apr 25.
7
Auxin-inducible protein depletion system in fission yeast.裂殖酵母中的生长素诱导蛋白消耗系统。
BMC Cell Biol. 2011 Feb 11;12:8. doi: 10.1186/1471-2121-12-8.
8
Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.裂殖酵母基因组复制起点的结构多样性与动力学
EMBO J. 2010 Mar 3;29(5):934-42. doi: 10.1038/emboj.2009.411. Epub 2010 Jan 21.
9
Molecular analysis of the replication program in unicellular model organisms.单细胞模式生物中复制程序的分子分析。
Chromosome Res. 2010 Jan;18(1):19-34. doi: 10.1007/s10577-009-9099-x.
10
Establishing the program of origin firing during S phase in fission Yeast.在裂殖酵母的S期建立起始点激发程序。
Cell. 2009 Mar 6;136(5):852-64. doi: 10.1016/j.cell.2009.01.017.
本文引用的文献
1
Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae.一种与酿酒酵母CDC6部分同源的新型CDC基因（ORC1）的特征分析。
Mol Biol Cell. 1996 Mar;7(3):409-18. doi: 10.1091/mbc.7.3.409.
2
A replication-enhancing element with transcriptional silencer activity in autonomously replicating human chromosomal fragments.在自主复制的人类染色体片段中具有转录沉默子活性的复制增强元件。
Mol Biol Cell. 1996 Jan;7(1):43-55. doi: 10.1091/mbc.7.1.43.
3
Large, complex modular structure of a fission yeast DNA replication origin.裂殖酵母DNA复制起点的大型复杂模块化结构。
Curr Biol. 1996 Apr 1;6(4):467-73. doi: 10.1016/s0960-9822(02)00514-6.
4
Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex.Orp1是S期调控因子Cdc18/Cdc6家族的成员，与复制起点识别复合体的一个组分同源。
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12475-9. doi: 10.1073/pnas.92.26.12475.
5
Genetic analysis of an ARS element from the fission yeast Schizosaccharomyces pombe.来自裂殖酵母粟酒裂殖酵母的一个自主复制序列元件的遗传分析。
EMBO J. 1995 Dec 15;14(24):6348-57. doi: 10.1002/j.1460-2075.1995.tb00326.x.
6
Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein.Cdc2和Cdc18与一种裂殖酵母ORC2样蛋白的相互作用。
Nature. 1996 Jan 25;379(6563):360-3. doi: 10.1038/379360a0.
7
Role for a Xenopus Orc2-related protein in controlling DNA replication.非洲爪蟾Orc2相关蛋白在控制DNA复制中的作用。
Nature. 1996 Jan 25;379(6563):357-60. doi: 10.1038/379357a0.
8
A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping.通过非随机序列标签位点作图构建的14兆碱基裂殖酵母基因组的13千碱基分辨率黏粒图谱。
Cell. 1993 Apr 9;73(1):121-32. doi: 10.1016/0092-8674(93)90165-m.
9
The silent P mating type locus in fission yeast contains two autonomously replicating sequences.裂殖酵母中的沉默P交配型基因座包含两个自主复制序列。
Nucleic Acids Res. 1993 Feb 25;21(4):855-61. doi: 10.1093/nar/21.4.855.
10
Eukaryotic DNA replication: anatomy of an origin.真核生物DNA复制：复制起点剖析
Annu Rev Biochem. 1993;62:29-63. doi: 10.1146/annurev.bi.62.070193.000333.
Zotero 插件