通过非平衡熔解实验评估嘧啶肽核酸与单链DNA靶标的结合情况。
Evaluation of pyrimidine PNA binding to ssDNA targets from nonequilibrium melting experiments.
作者信息
Lesnik E A, Risen L M, Driver D A, Griffith M C, Sprankle K, Freier S M
机构信息
ISIS Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA.
出版信息
Nucleic Acids Res. 1997 Feb 1;25(3):568-74. doi: 10.1093/nar/25.3.568.
Abstract
Slow kinetics of homopyrimidine PNA binding to single stranded DNA and RNA targets is manifested in significant hysteresis in thermal UV absorption experiments. We have compared temperatures of dissociation (Tdis) and reassociation (Tass) for triplexes formed by DNA and single or bis PNAs with K50 derived from gel mobility experiments. Results indicated there was no correlation between Tdis and K50 while reasonable correlation between Tass and K50 was found. This correlation enabled use of easy thermal UV absorption experiments for evaluation of PNA binding to DNA/RNA targets.
摘要
同嘧啶肽核酸（PNA）与单链DNA和RNA靶标的结合动力学缓慢，这在热紫外吸收实验中表现为显著的滞后现象。我们比较了由DNA与单链或双链PNA形成的三链体的解离温度（Tdis）和重新结合温度（Tass），并通过凝胶迁移实验得出了K50。结果表明，Tdis与K50之间没有相关性，而Tass与K50之间存在合理的相关性。这种相关性使得利用简单的热紫外吸收实验来评估PNA与DNA/RNA靶标的结合成为可能。
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2
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Science. 1995 Dec 15;270(5243):1838-41. doi: 10.1126/science.270.5243.1838.
3
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4
Peptide nucleic acid (PNA) conformation and polymorphism in PNA-DNA and PNA-RNA hybrids.肽核酸（PNA）在PNA-DNA和PNA-RNA杂交体中的构象与多态性。
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