I组内含子RNA自我剪接的抑制作用：高通量筛选试验
Inhibition of self-splicing group I intron RNA: high-throughput screening assays.
作者信息
Mei H Y, Cui M, Sutton S T, Truong H N, Chung F Z, Czarnik A W
机构信息
Department of Chemistry, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48106, USA.
出版信息
Nucleic Acids Res. 1996 Dec 15;24(24):5051-3. doi: 10.1093/nar/24.24.5051.
Abstract
High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenable to high-throughput screening have been established. Small molecules that inhibit the functions of group I introns have been identified. These inhibitors should be useful in better understanding ribozyme catalysis or in therapeutic intervention of group I intron-containing microorganisms.
摘要
高通量筛选分析方法已被开发出来，用于快速鉴定靶向催化I组内含子的小分子抑制剂。对源自卡氏肺孢子虫或噬菌体T4的自我剪接I组内含子催化的生化反应进行了研究。已建立了适用于高通量筛选的体外生化分析方法。已鉴定出抑制I组内含子功能的小分子。这些抑制剂应有助于更好地理解核酶催化作用，或对含I组内含子的微生物进行治疗干预。
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Inhibition of in vitro splicing of a group I intron of Pneumocystis carinii.卡氏肺孢子虫I组内含子体外剪接的抑制作用。
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