针对鼠乳腺肿瘤病毒36000道尔顿糖蛋白的放射免疫测定显示了类型、组和种间决定簇。
Radioimmunoassays for the 36,000-dalton glycoprotein of murine mammary tumor viruses demonstrate type, group, and interspecies determinants.
作者信息
Teramoto Y A, Schlom J
出版信息
J Virol. 1979 Aug;31(2):334-40. doi: 10.1128/JVI.31.2.334-340.1979.
Mouse mammary tumor viruses (MMTVs) contain distinct membrane glycoproteins of 52,000 daltons (gp52) and 36,000 daltons (gp36). We report here the development of new radioimmunoassays for gp36, using gp36 purified by hydrophobic chromatography and gel filtration. These assays demonstrate that gp36 has both type-specific and group-specific antigenic determinants. The virus-coded nature of these determinants was shown by utilizing different MMTVs grown in the same feline cell line. Interspecies determinants on gp36 were demonstrated by the observations that (i) MC-MTV (a virus isolate from the Asian rodent Mus cervicolor, and morphologically identical to MMTVs) competed, with an altered slope, in the gp36 radioimmunoassay, and (ii) antisera raised against MC-MTV immunopreciptitated 125I-labeled gp36. The detection of gp36 in spontaneous mammary tumors of several strains of mice also facilitates further studies on the replication of MMTVs and the host's immune response to MMTV-mediated oncogenesis.
小鼠乳腺肿瘤病毒（MMTVs）含有分子量分别为52,000道尔顿（gp52）和36,000道尔顿（gp36）的独特膜糖蛋白。我们在此报告了使用经疏水层析和凝胶过滤纯化的gp36开发gp36新放射免疫测定法的情况。这些测定法表明，gp36具有型特异性和组特异性抗原决定簇。利用在同一猫细胞系中生长的不同MMTVs证明了这些决定簇的病毒编码性质。通过以下观察结果证明了gp36上的种间决定簇：（i）MC-MTV（一种从亚洲啮齿动物中华姬鼠分离的病毒，在形态上与MMTVs相同）在gp36放射免疫测定中以改变的斜率竞争，以及（ii）针对MC-MTV产生的抗血清免疫沉淀了125I标记的gp36。在几种小鼠品系的自发性乳腺肿瘤中检测gp36也有助于进一步研究MMTVs的复制以及宿主对MMTV介导的肿瘤发生的免疫反应。
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