Litwińska B, Biesiadecka A, Kańtoch M
Department of Virology, National Institute of Hygiene, Warsaw, Poland.
Arch Immunol Ther Exp (Warsz). 1996;44(5-6):379-83.
The temperature mutants of herpes simplex virus type 1 has been received. The following properties on different levels of passages were investigated: stability of virulence, virus infection titer and immunogenicity. The virulence stability was expressed by percent of survivors and mean survival time in infected mice (CFW/Pzh and BALB/cPzh strains). Virus infection titer's stability was measured after prolonged exposure of temperature sensitive mutant (ts 28 degrees C) to critical temperatures (+4 degrees C and from +20 degrees C to +23 degrees C). Stability of immunogenic potency of ts 28 degrees C was evaluated in mice immunized with the virus on different level of passages and after that they were challenged with highly pathogenic temperature resistant clone (tr 39 degrees C). The performed experiments proved the stability of investigated features.
已获得1型单纯疱疹病毒的温度突变体。对不同传代水平下的以下特性进行了研究：毒力稳定性、病毒感染滴度和免疫原性。毒力稳定性通过感染小鼠（CFW/Pzh和BALB/cPzh品系）的存活百分比和平均存活时间来表示。在温度敏感突变体（ts 28℃）长时间暴露于临界温度（+4℃以及从+20℃至+23℃）后，测量病毒感染滴度的稳定性。在不同传代水平用该病毒免疫小鼠，然后用高致病性耐温克隆株（tr 39℃）攻击，以此评估ts 28℃免疫原效力的稳定性。所进行的实验证明了所研究特性的稳定性。
