Litwińska B, Trzcińska A, Kańtoch M
Zakład Wirusologii Państwowego Zakładu Higieny w Warszawie.
Med Dosw Mikrobiol. 2001;53(1):71-87.
The aim of the study was to characterize biological features of the sensitive mutant of HSV-1, derived from McIntyre strain by numerous virus passages at lowered replication temperature (28 degrees C). Pathogenicity of obtained ts mutant for inbred mice lines, CFW/Pzh and BALB/cPzh, was determined. Statistically significant decrease in virulence of the mutant for these mouse lines was demonstrated, as compared with the native virus strain, propagated at 37 degrees C. Immunogenic activity of ts mutant of HSV-1 defined by the possibility of mouse protection against infection with high virulent was determined. Mice, which at the time of immunization with ts mutant received Depo-Medrol--an immunosuppressive agent--were also found to be capable of inducing defense mechanisms to infection with the native strain.
本研究的目的是表征单纯疱疹病毒1型（HSV-1）敏感突变体的生物学特性，该突变体由麦金太尔毒株在较低复制温度（28摄氏度）下多次传代获得。测定了所得温度敏感（ts）突变体对近交系小鼠CFW/Pzh和BALB/cPzh的致病性。结果表明，与在37摄氏度下繁殖的天然病毒株相比，该突变体对这些小鼠品系的毒力有统计学意义的降低。通过小鼠抵御高毒力感染的可能性来确定HSV-1的ts突变体的免疫原活性。还发现，在用ts突变体免疫时接受了免疫抑制剂甲泼尼龙的小鼠，也能够诱导针对天然毒株感染的防御机制。
