Korycka A, Robak T
Department of Pharmacology, Medical Academy, Lódź, Poland.
Arch Immunol Ther Exp (Warsz). 1996;44(4):259-64.
The objective of the present study was to investigate the interactions of 2-chlorodeoxyadenosine (2-CdA) with interferon alpha or gamma (IFN-alpha, IFN-gamma), as well as between 2-CdA and recombinant human tumor necrosis factor alpha (rhTNF-alpha), on the clonal growth of granulocyte-macrophage progenitor cells (CFU-GM) from patients with chronic myeloid leukemia (CML) and on clonogenic leukemia blasts (CFU-L) from acute myeloid leukemia (AML) patients. Progenitor cell culture in semisolid medium in vitro was applied and the percentage of colony growth inhibition was evaluated. The use of 2-CdA either with IFN-alpha or IFN-gamma and 2-CdA with TNF-alpha was found to inhibit, in a dose dependent manner, the growth of colonies formed by hematopoietic precursor cells from CML and AML patients as well as from normal individuals, with the greatest effect being observed after the use of 2-CdA and IFN-alpha at their highest concentrations.
本研究的目的是探讨2-氯脱氧腺苷（2-CdA）与α干扰素或γ干扰素（IFN-α、IFN-γ）之间的相互作用，以及2-CdA与重组人肿瘤坏死因子α（rhTNF-α）之间的相互作用，对慢性粒细胞白血病（CML）患者的粒细胞-巨噬细胞祖细胞（CFU-GM）克隆生长以及急性髓细胞白血病（AML）患者的克隆性白血病母细胞（CFU-L）的影响。采用体外半固体培养基中的祖细胞培养，并评估集落生长抑制百分比。发现2-CdA与IFN-α或IFN-γ联合使用以及2-CdA与TNF-α联合使用，均以剂量依赖性方式抑制CML和AML患者以及正常个体的造血前体细胞形成的集落生长，在使用2-CdA和IFN-α的最高浓度后观察到最大效果。
