纤细裸藻光感受器的体内光循环。
In vivo photocycle of the Euglena gracilis photoreceptor.
作者信息
Barsanti L, Passarelli V, Walne P L, Gualtieri P
机构信息
Istituto di Biofisica, CNR, Pisa, Italy.
出版信息
Biophys J. 1997 Feb;72(2 Pt 1):545-53. doi: 10.1016/s0006-3495(97)78694-6.
We present the light-induced photocycle of the paraflagellar swelling of Euglena gracilis. The kinetics of this process was reconstructed by sampling its fluorescence emission and switching the excitation light from 365 nm to 436 nm. Stable intermediates in the photocycle were manifested. The measured millisecond resolution kinetics best fits a Michaelis-Menten equation. The data provide strong evidence that the paraflagellar swelling, a three-dimensional natural crystal of a light-detecting protein, is the true Euglena photoreceptor.
我们展示了纤细裸藻副鞭毛肿胀的光诱导光循环。通过对其荧光发射进行采样并将激发光从365纳米切换到436纳米，重构了该过程的动力学。光循环中的稳定中间体得以显现。所测得的毫秒级分辨率动力学最符合米氏方程。这些数据提供了强有力的证据，表明副鞭毛肿胀，一种光探测蛋白的三维天然晶体，是真正的裸藻光感受器。
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