Masnatta L D, Cuniberti L A, Rey R H, Werba J P
Basic Sciences Research Institute (HCB), Dr. René G. Favaloro University Foundation, Solis, Buenos Aires, Argentina.
J Chromatogr B Biomed Appl. 1996 Dec 13;687(2):437-42. doi: 10.1016/s0378-4347(96)00254-x.
A selective high-performance liquid chromatographic method to assess either bezafibrate, ciprofibrate or fenofibric acid plasma levels is described. Drugs are extracted with diethyl ether, after acidification with HCL. An isocratic acetonitrile 0.02 M H3PO4 (55:45) mobile phase, a C18 microns) column and UV detection are used. The LOQ found was 0.25 microgram/ml for the three fibrates. Intra- and inter-assay accuracy ranges were 90-107% and 82-111%: 96-115% and 94-107%: 94-114% and 94-126% for bezafibrate, ciprofibrate and fenofibric acid, respectively. Intra- and inter-assay precision (C.V.% ranges) were 1.72-3.06% and 2.66-7.67%: 1.88-4.64% and 0.62-2.99%: 1.26-4.69% and 3.56-7.17% for the three fibrates studied. Its sensitivity, accuracy and precision make it a useful tool for monitoring plasma levels of these drugs in a clinical setting and for research purposes.
本文描述了一种用于评估苯扎贝特、环丙贝特或非诺贝特酸血浆水平的选择性高效液相色谱法。用盐酸酸化后，药物用乙醚萃取。使用等度乙腈-0.02M磷酸（55:45）流动相、C18（微米）柱和紫外检测。三种贝特类药物的定量限均为0.25微克/毫升。苯扎贝特、环丙贝特和非诺贝特酸的批内和批间准确度范围分别为90-107%和82-111%、96-115%和94-107%、94-114%和94-126%。所研究的三种贝特类药物的批内和批间精密度（变异系数范围）分别为1.72-3.06%和2.66-7.67%、1.88-4.64%和0.62-2.99%、1.26-4.69%和3.56-7.17%。其灵敏度、准确度和精密度使其成为临床环境中监测这些药物血浆水平以及用于研究目的的有用工具。
