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在存在和不存在δ1-四氢大麻酚的情况下大鼠肝脏微粒体的差示扫描量热法和酶活性

Differential scanning calorimetry and enzymic activity of rat liver microsomes in the presence and absence of delta1-tetrahydrocannabinol.

作者信息

Bach D, Bursuker I, Goldman R

出版信息

Biochim Biophys Acta. 1977 Sep 5;469(2):171-9. doi: 10.1016/0005-2736(77)90179-1.

DOI:10.1016/0005-2736(77)90179-1
PMID:901781
Abstract

The thermal transitions of rat liver microsomes and isolated lipids were investigated by using differential scanning calorimetry. Endothermic transitions at approximately-5 degrees C and between approximately18 degrees and 40 degrees C were detected in the membranes and at approximately-10 degrees C and between approximately 10 degress and 20 degrees C in the extracted lipids. Interaction with delta1-tetrahydrocannabinol of microsomal membranes and of extracted lipids influences the thermotrophic behaviour as revealed by differential scanning calorimetry and eliminates the break in the Arrhenius plot of the enzymic activity of O-demethylase.

摘要

采用差示扫描量热法研究了大鼠肝脏微粒体和分离脂质的热转变。在膜中检测到约-5℃以及约18℃至40℃之间的吸热转变,在提取的脂质中检测到约-10℃以及约10℃至20℃之间的吸热转变。差示扫描量热法显示,微粒体膜和提取脂质与δ1-四氢大麻酚的相互作用会影响热营养行为,并消除O-脱甲基酶酶活性的阿伦尼乌斯图中的断点。

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引用本文的文献

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Biochem J. 1997 Mar 1;322 ( Pt 2)(Pt 2):435-40. doi: 10.1042/bj3220435.
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Differential scanning calorimetry of rat liver mitochondria.大鼠肝脏线粒体的差示扫描量热法
Experientia. 1978 Jun 15;34(6):717-8. doi: 10.1007/BF01947277.
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Differential scanning calorimetry of chromaffin granule membranes.嗜铬粒细胞膜的差示扫描量热法
Experientia. 1979 Jun 15;35(6):750-1. doi: 10.1007/BF01968223.