Akhrem A A, Kisel M A, Lunevich A Y, Bokut S B, Andrianov V T, Kiselev P A
FEBS Lett. 1983 Dec 12;164(2):247-50. doi: 10.1016/0014-5793(83)80295-6.
The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg2+. Addition of Mg2+ yields three partially reversible phase transitions at 18, 27 and 32 degrees C, respectively. A character of Mg2+-induced rearrangements in a membrane and their relation to a catalytic function of a cytochrome P-450-dependent enzymatic system is discussed.
采用差示扫描量热法以及使用探针ANS和芘的荧光法，研究了有无Mg2+存在时大鼠肝脏微粒体的热致行为。添加Mg2+分别在18、27和32摄氏度时产生三个部分可逆的相变。讨论了Mg2+诱导的膜重排特征及其与细胞色素P-450依赖性酶系统催化功能的关系。
