Yalçin O, Soybir G, Köksoy F, Köse H, Oztürk R, Cokneşeli B
Department of Surgery, Istanbul Taksim Hospital, Turkey.
Surg Today. 1997;27(2):154-8. doi: 10.1007/BF02385906.
The presence of certain defects in both cellular and humoral immunity after thermal injury has been established. Likewise, the translocation of enteric bacteria to the mesenteric lymph nodes and to distant organs has also been observed following serious thermal injury. The effects of granulocyte colony-stimulating factor (G-CSF) on bacterial translocation, the small bowel mucosa, and cecal bacterial content were investigated in a rat model of burn wound sepsis in which albino Wistar rats were scalded over 30% of their bodies, after which the lesions were infected by 1 x 10(8) colony-forming units (cfu) Pseudomonas aeruginosa. The control group was treated with 5% dextrose solution subcutaneously starting 2 days preburn, while the treatment group received 100 microg/kg human G-CSF subcutaneously. On the 4th day post burn all animals were killed to examine the bowel and culture of the mesenteric lymph nodes (MLN), livers, and spleens. No significant differences were observed between the groups regarding the cecal bacterial content and small bowel; however, a difference was seen in the ratio of translocation in the MLN liver and spleen and quantitative MLN cultures. Based on these findings, G-CSF was thus found to be significantly effective in reducing bacterial translocation due to burn wound sepsis.
热损伤后细胞免疫和体液免疫中某些缺陷的存在已得到证实。同样，严重热损伤后也观察到肠道细菌向肠系膜淋巴结和远处器官的移位。在烧伤创面脓毒症大鼠模型中，研究了粒细胞集落刺激因子（G-CSF）对细菌移位、小肠黏膜和盲肠细菌含量的影响。该模型中，白化Wistar大鼠身体30%被烫伤，然后损伤部位被1×10⁸ 菌落形成单位（cfu）的铜绿假单胞菌感染。对照组在烧伤前2天开始皮下注射5%葡萄糖溶液，而治疗组皮下注射100μg/kg人G-CSF。烧伤后第4天处死所有动物，检查肠道并对肠系膜淋巴结（MLN）、肝脏和脾脏进行培养。两组在盲肠细菌含量和小肠方面未观察到显著差异；然而，在MLN、肝脏和脾脏中的移位率以及MLN定量培养方面存在差异。基于这些发现，结果表明G-CSF在减少烧伤创面脓毒症引起的细菌移位方面具有显著效果。
