Dorsman J C, Teunisse A F, Zantema A, van der Eb A J
Laboratory for Molecular Carcinogenesis, Department of Medical Biochemistry, Leiden University, Wassenaarseweg, The Netherlands.
J Gen Virol. 1997 Feb;78 ( Pt 2):423-6. doi: 10.1099/0022-1317-78-2-423.
The cellular transcription co-activators p300 and the CREB-binding protein CBP are cellular targets for transformation by the E1A proteins of non-oncogenic adenovirus 5 (Ad5). In this study, we show that the E1A proteins of oncogenic Ad12, like those of Ad5, can also bind to CBP and that this interaction is direct. In addition, we show that the Ad12 E1A proteins can also bind directly to p300. These results suggest that E1A can modulate the function of proteins of the p300 family via direct protein-protein interactions.
细胞转录共激活因子p300和CREB结合蛋白CBP是无致癌性腺病毒5（Ad5）的E1A蛋白介导细胞转化的作用靶点。在本研究中，我们发现致癌性Ad12的E1A蛋白与Ad5的E1A蛋白一样，也能与CBP结合，且这种相互作用是直接的。此外，我们还发现Ad12的E1A蛋白也能直接与p300结合。这些结果表明，E1A可通过直接的蛋白质-蛋白质相互作用来调节p300家族蛋白的功能。
