Mannervik M, Akusjärvi G
Department of Medical Immunology and Microbiology, BMC, Uppsala University, Sweden.
FEBS Lett. 1997 Sep 1;414(1):111-6. doi: 10.1016/s0014-5793(97)00982-4.
p300 and CBP are two related transcriptional co-activator proteins required by many cellular transcription factors for activity. The adenovirus E1A protein binds p300 and CBP through its amino-terminus and conserved region (CR) 1. Fusing CR1 to a heterologous DNA-binding domain creates a potent transcriptional activator, suggesting that CR1 might activate transcription by recruiting p300/CBP to the promoter. We show that both p300 and CBP enhances CR1-dependent transactivation. However, this enhancement occurs independently of a direct interaction with E1A and does not correlate with the CR1 activator function.
p300和CBP是两种相关的转录共激活蛋白，许多细胞转录因子发挥活性都需要它们。腺病毒E1A蛋白通过其氨基末端和保守区域（CR）1与p300和CBP结合。将CR1与异源DNA结合结构域融合可产生一种强效转录激活因子，这表明CR1可能通过将p300/CBP募集到启动子来激活转录。我们发现p300和CBP均可增强CR1依赖性反式激活。然而，这种增强独立于与E1A的直接相互作用发生，并且与CR1激活因子功能无关。
