Mitchen J, Oberley T, Wilding G
Department of Medicine, University of Wisconsin Clinical Cancer Center, Madison, USA.
Prostate. 1997 Jan 1;30(1):7-19. doi: 10.1002/(sici)1097-0045(19970101)30:1<7::aid-pros2>3.0.co;2-n.
Continuous culturing of two distinct human prostate specimens in the presence of interstitial collagenase added directly to conventional medium resulted in the isolation and extended growth of primary epithelial prostate cell (PEPC) cultures from each. Both continued to proliferate substantially beyond the average time determined for analogous untreated epithelial prostate isolates. Both repeatedly stain positive for keratin and are characteristically epithelial in morphological appearance and growth model. Both express androgen receptor mRNA and stain positive for androgen receptors. PEPC-2 displays an androgen dose-dependent stimulation of cell proliferation, as well as specifically binding 3H-R1881. PEPC-1 exhibits a hypotetraploid karyotype with loss of the Y chromosome. PEPC-2 conserves a normal human ploidy, including the Y chromosome, although there is extensive random chromosome loss. Elimination of the collagenase from the medium resulted in decreased cellular proliferation and accumulation of stainable collagen in both PEPC cultures. Neither PEPC isolate produced tumors in male nude mice, whether injected alone, mixed with matrigel, or combined with prostate or bone fibroblastic cells.
将间质胶原酶直接添加到传统培养基中，对两种不同的人类前列腺标本进行连续培养，从而从每种标本中分离出原代前列腺上皮细胞（PEPC）培养物并使其长期生长。两者的增殖时间都大大超过了未经处理的类似前列腺上皮分离物所确定的平均时间。两者对角蛋白均反复呈阳性染色，在形态外观和生长模式上均具有典型的上皮特征。两者均表达雄激素受体mRNA，且雄激素受体呈阳性染色。PEPC-2表现出雄激素剂量依赖性的细胞增殖刺激作用，并能特异性结合³H-R1881。PEPC-1呈现亚四倍体核型，Y染色体缺失。PEPC-2保持正常的人类倍性，包括Y染色体，尽管存在广泛的随机染色体丢失。从培养基中去除胶原酶会导致两种PEPC培养物中的细胞增殖减少以及可染色胶原的积累。无论是单独注射、与基质胶混合注射还是与前列腺或骨成纤维细胞联合注射，两种PEPC分离物在雄性裸鼠中均未产生肿瘤。
