采用胶束电动毛细管色谱法测定红细胞中的5-氨基乙酰丙酸脱水酶活性及尿液中的胆色素原。

Determination of 5-aminolaevulinic acid dehydratase activity in erythrocytes and porphobilinogen in urine by micellar electrokinetic capillary chromatography.

作者信息

Luo J L, Deka J, Lim C K

机构信息

MRC Toxicology Unit, University of Leicester, UK.

出版信息

J Chromatogr A. 1996 Jan 26;722(1-2):353-7. doi: 10.1016/0021-9673(95)00723-7.

DOI:10.1016/0021-9673(95)00723-7

PMID:9019304

Abstract

A micellar electrokinetic capillary chromatographic (MECC) method has been developed and optimised for the separation of 5-aminolaevulinic acid (ALA) and porphobilinogen (PBG). The running buffer consisted of a mixture of 20 mM sodium phosphate and 20 mM sodium borate containing 50 mM sodium dodecyl sulphate (SDS) adjusted to pH 9.5 with 1 M NaOH. The running voltage and temperature were 20-25 kV and 30 degrees C, respectively. The MECC method for the analysis of PBG is fast and simple and is useful for the screening of PBG in the urine of patients suspected to have acute intermittent porphyria (AIP), and for the confirmation of lead exposure by measuring red-cell ALA-dehydratase (ALA-D) activity with ALA as the enzyme substrate.

摘要

已开发并优化了一种胶束电动毛细管色谱（MECC）方法，用于分离5-氨基乙酰丙酸（ALA）和胆色素原（PBG）。运行缓冲液由20 mM磷酸钠和20 mM硼酸钠的混合物组成，其中含有50 mM十二烷基硫酸钠（SDS），用1 M NaOH调节至pH 9.5。运行电压和温度分别为20 - 25 kV和30℃。用于分析PBG的MECC方法快速简便，可用于筛查疑似患有急性间歇性卟啉症（AIP）患者尿液中的PBG，以及通过以ALA作为酶底物测量红细胞ALA脱水酶（ALA-D）活性来确认铅暴露情况。