Noguchi E, Shibasaki M, Isoyama S, Takita H
Department of Pediatrics, Institute of Clinical Medicine, University of Tsukuba, Japan.
Allergy. 1996 Dec;51(12):907-13. doi: 10.1111/j.1398-9995.1996.tb04491.x.
Although asthmatic children are often sensitized to the house-dust mite (HDM) during early childhood, it is not clear what antigenic components are associated with the early immune response of these children. To investigate this problem, we evaluated IgE, IgG, and IgG4 antibody responses to Dermatophagoides farinae (Df) by immunoblotting among three groups of children: group I aged 0-4 years, group II aged 5-9 years, and group III aged 10-14 years. In the group I subjects, positive IgE-binding reactions to 15- and 25-kDa components were found in 76% and 44% of sera, respectively. Those to other components were generally low in frequency. In contrast, positive IgG-binding reactions to 15- and 25-kDa components were found in 44% and 24% of sera, and those to 30- and 110-kDa components in 48% and 88% of sera, respectively. Positive IgG4 reactions to 15- and 25-kDa components were found in 48% and 24%, respectively, and those to other components were very low. Positive IgE-binding reactions to these components gradually became more frequent with increasing age in groups II and III, while IgG and IgG4 reactivities were not markedly different in these age groups. These results suggest that the 15- and 25-kDa proteins of Df are important antigens associated with the initial IgE response to HDM in early childhood, while the 30- and 110-kDa proteins are associated with IgG and 15-kDa components with IgG4.
尽管哮喘儿童在幼儿期常对屋尘螨（HDM）致敏，但尚不清楚哪些抗原成分与这些儿童的早期免疫反应相关。为了研究这个问题，我们通过免疫印迹法评估了三组儿童对粉尘螨（Df）的IgE、IgG和IgG4抗体反应：第一组年龄为0 - 4岁，第二组年龄为5 - 9岁，第三组年龄为10 - 14岁。在第一组受试者中，分别在76%和44%的血清中发现了对15 kDa和25 kDa成分的阳性IgE结合反应。对其他成分的反应频率普遍较低。相比之下，分别在44%和24%的血清中发现了对15 kDa和25 kDa成分的阳性IgG结合反应，在48%和88%的血清中发现了对30 kDa和110 kDa成分的阳性IgG结合反应。对15 kDa和25 kDa成分的阳性IgG4反应分别为48%和24%，对其他成分的反应非常低。在第二组和第三组中，随着年龄增长，对这些成分的阳性IgE结合反应逐渐变得更加频繁，而这些年龄组中的IgG和IgG4反应性没有明显差异。这些结果表明，Df的15 kDa和25 kDa蛋白是与幼儿期对HDM的初始IgE反应相关的重要抗原，而30 kDa和110 kDa蛋白与IgG相关，15 kDa成分与IgG4相关。
