利用肾切片评估螯合剂从肾脏中去除汞的效果。

Utilization of renal slices to evaluate the efficacy of chelating agents for removing mercury from the kidney.

作者信息

Keith R L, Setiarahardjo I, Fernando Q, Aposhian H V, Gandolfi A J

机构信息

Center for Toxicology, The University of Arizona, Tucson 85721, USA.

出版信息

Toxicology. 1997 Jan 15;116(1-3):67-75. doi: 10.1016/s0300-483x(96)03531-7.

DOI:10.1016/s0300-483x(96)03531-7

PMID:9020508

Abstract

Mercury is an environmental contaminant that preferentially accumulates in the kidney. It has been previously shown using proton-induced X-ray emission analysis that mercury (HgCl2) accumulated in precision-cut rabbit renal cortical slices. In this study, the efficacy of seven chelating agents for the removal of Hg from renal slices has been examined. Rabbits were injected with HgCl2 (10 mg/kg) and 3 h later kidneys were sliced, or renal slices were exposed in vitro to a mildly toxic concentration of HgCl2 (5 x 10(-5)M, 4 h). The slices were then treated in vitro with 10 mM concentrations of EDTA, lipoic acid (LA), penicillamine (PA), glutathione (GSH), 1,4-dithiothreitol (DTT), DMSA, or DMPS. DMPS proved to be the most effective in mobilizing Hg from in vivo or in vitro HgCl2-exposed renal tissue ( > 85% of control after 3 h incubation). Relative efficacies for the seven agents were DMPS > DMSA, PA > DTT, GSH > LA, EDTA. The use of renal slices appears to be a useful in vitro tool for assessing the efficacy of chelating agents on mobilizing accumulated Hg from renal tissue.

摘要

汞是一种环境污染物，它优先在肾脏中蓄积。先前通过质子诱导X射线发射分析表明，汞（HgCl₂）在精切的兔肾皮质切片中蓄积。在本研究中，已检测了七种螯合剂从肾切片中去除汞的效果。给兔子注射HgCl₂（10 mg/kg），3小时后将肾脏切片，或者将肾切片在体外暴露于轻度毒性浓度的HgCl₂（5×10⁻⁵M，4小时）。然后将切片在体外用10 mM浓度的乙二胺四乙酸（EDTA）、硫辛酸（LA）、青霉胺（PA）、谷胱甘肽（GSH）、1,4-二硫苏糖醇（DTT）、二巯基丁二酸（DMSA）或二巯基丙磺酸钠（DMPS）进行处理。事实证明，DMPS在从体内或体外HgCl₂暴露的肾组织中动员汞方面最有效（孵育3小时后，超过对照组的85%）。这七种试剂的相对有效性为DMPS＞DMSA，PA＞DTT，GSH＞LA，EDTA。使用肾切片似乎是一种有用的体外工具，可用于评估螯合剂从肾组织中动员蓄积汞的效果。