Nucleotide-sugar binding to ribosomes. Comparison of affinity and capacity of gastric mucosa and liver ribosomes to sugar-nucleotides.

Highly purified ribosomes from gastric mucosa and liver were isolated and tested for their affinity and capacity toward UDP- monosaccharide binding. Ribosomes from both tissues bind UDP-N-acetylglucosamine nearly at the same level with similar affinity, but binding capacity of gastric ribosomes to UDP-N-acetylgalactosamine was twice higher than in case of liver ribosomes, and with higher affinity than in case of UDP-N-acetylglucosamine. The binding of UDP-N-acetylgalactosamine to mucosa ribosomes requires the whole sugar-nucleotide structure.