Pepiński W, Skawrońska M, Janica J
Department of Forensic Medicine, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1996;41(2):316-20.
Population genetic studies within VNTR locus D1S80 were carried out on a population sample from the north-eastern Poland. DNA extraction was performed using the Chelex method. DNA amplification was followed by high-resolution PAGE and silver staining (AMP-FLP technique). In 116 unrelated individuals 20 alleles were observed. The D1S80 locus demonstrated a heterozygosity of 0.85. Comparison of the observable allele frequencies with population data from other Caucasian populations revealed no significant differences.
在位于可变数目串联重复序列(VNTR)位点D1S80上,对来自波兰东北部的人群样本进行了群体遗传学研究。使用Chelex方法进行DNA提取。DNA扩增后采用高分辨率聚丙烯酰胺凝胶电泳(PAGE)和银染法(扩增片段长度多态性技术,AMP-FLP)。在116名无亲缘关系的个体中观察到20个等位基因。D1S80位点的杂合度为0.85。将观察到的等位基因频率与其他高加索人群的群体数据进行比较,未发现显著差异。