Skrzydlewska E
Department of Instrumental Analysis, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1996;41(2):397-404.
The liver is the main metabolic place where the methanol is oxidized to formaldehyde and to formate. The aim of this paper was to study the liver antioxidant system in acute methanol intoxication, after 6, 12, 24 hours and 2, 5 and 7 days of alcohol administration into rats. In liver homogenates the superoxide dismutase, catalase, peroxidase and reductase glutathione activity and content of malondialydehyde (MDA), SH-compounds in protein and non-protein fraction and ascorbate were estimated. Activity of superoxide dismutase and catalase was significantly increased after 6 hours following methanol ingestion and persisted up to 2-5 days of intoxication. It was accompanied by significant decreased of reductase and peroxidase glutathione activities. The protein and non-protein SH-groups were significantly decreased during 6 hours to 5 days following methanol ingestion. The liver MDA content was considerably increased. After 2 days since methanol intoxication the liver vitamin C content was significantly decreased in comparison with the control group. The obtain results demonstrated that during methanol induced liver injury there are increase of lipid peroxidation and impairment of proantioxidant equilibrium in favour to prooxidant.
肝脏是甲醇氧化为甲醛和甲酸的主要代谢场所。本文旨在研究给大鼠灌胃酒精6、12、24小时以及2、5和7天后急性甲醇中毒时肝脏的抗氧化系统。测定了肝脏匀浆中超氧化物歧化酶、过氧化氢酶、过氧化物酶和还原型谷胱甘肽还原酶的活性以及丙二醛（MDA）的含量、蛋白质和非蛋白质组分中的SH化合物含量以及抗坏血酸含量。摄入甲醇6小时后，超氧化物歧化酶和过氧化氢酶的活性显著增加，并持续到中毒后的2至5天。同时，还原型谷胱甘肽还原酶和过氧化物酶的活性显著降低。摄入甲醇后6小时至5天，蛋白质和非蛋白质的SH基团显著减少。肝脏MDA含量显著增加。甲醇中毒2天后，与对照组相比，肝脏维生素C含量显著降低。所得结果表明，在甲醇诱导的肝损伤过程中，脂质过氧化增加，抗氧化剂平衡受损，有利于促氧化剂。
