Skrzydlewska E, Farbiszewski R
Department of Analytical Chemistry, Medical Academy, Bialystok, Poland.
Vet Hum Toxicol. 1996 Dec;38(6):429-33.
The activities of superoxide dismutase (SOD), catalase glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-R) and the concentration of ascorbate, alpha-tocopherol, non-protein and protein-bound sulfhydryl compounds, and thiobarbituric acid-reactive substances (TBA-rs) in liver, erythrocytes and serum of rats dosed with 1.5 g methanol/kg bw were measured after 6, 12 and 24 h and 2, 5 and 7 d. Hematological erythrocyte parameters were also determined. Liver GSH-Px and GSSG-R activities, SH-groups and ascorbate were significantly diminished at 12 and 24 h, while TBA-rs increased. Blood SOD, GSH-Px and GSSG-R activities and sulfhydryl-group concentrations were reduced while TBA-rs were elevated. Methanol given to rats impaired liver, erythrocyte and blood serum antioxidant mechanisms.
给大鼠按1.5克甲醇/千克体重的剂量给药后，在6、12和24小时以及2、5和7天时，测定了肝脏、红细胞和血清中超氧化物歧化酶（SOD）、过氧化氢酶、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽还原酶（GSSG-R）的活性，以及抗坏血酸、α-生育酚、非蛋白结合和蛋白结合巯基化合物、硫代巴比妥酸反应性物质（TBA-rs）的浓度。还测定了血液学红细胞参数。肝脏GSH-Px和GSSG-R活性、SH基团和抗坏血酸在12和24小时时显著降低，而TBA-rs增加。血液中SOD、GSH-Px和GSSG-R活性以及巯基浓度降低，而TBA-rs升高。给大鼠服用甲醇会损害肝脏、红细胞和血清的抗氧化机制。
