Niczyporuk W, Krajewska-Kułak E, Zimnoch L
Department of Dermatology and Venereology, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1996;41(2):515-24.
The aim of the study was to make a selection of the calmodulin antagonists, which could be useful in topical treatment of psoriasis. Four drugs were assessed: chlorpromazine, trifluoperazine, miconazole and ketoconazole. They were applied on the skin of guinea pigs, twice a day for the period of two weeks. Biopsy specimens from these sites were being taken for light microscopy, histoenzymatic examination and for evaluation of the proliferation activity of the epidermis (the AgNOR stain, after Ploton). A decrease in reaction activities for lactate dehydrogenase and succinic dehydrogenase as well as in the proliferation activity of the epidermis, could suggest an inhibitory effect of chlorpromazine and miconazole on the cell cycle and keratinization process.
该研究的目的是筛选出可用于银屑病局部治疗的钙调蛋白拮抗剂。评估了四种药物：氯丙嗪、三氟拉嗪、咪康唑和酮康唑。将它们每天两次涂抹在豚鼠皮肤上，持续两周。从这些部位采集活检标本用于光学显微镜检查、组织酶学检查以及评估表皮的增殖活性（采用普罗顿法进行AgNOR染色）。乳酸脱氢酶和琥珀酸脱氢酶的反应活性降低以及表皮增殖活性降低，可能表明氯丙嗪和咪康唑对细胞周期和角质化过程具有抑制作用。
