Solheim E, Pinholt E M, Bang G, Sudmann E
Institute for Surgical Research, University of Oslo, Norway.
J Craniofac Surg. 1995 May;6(3):195-8. doi: 10.1097/00001665-199505000-00004.
It has been shown that different sterilization procedures of demineralized bone may influence its osteoinductive properties. The aim of this study was to evaluate the effect of ethylene oxide sterilization for 1, 3, and 6 hours on the osteoinductive potential of allogeneic demineralized bone implanted heterotopically in rats. Sixty male Wistar rats were randomly assigned to one of four groups, A through D, and four demineralized bone chips (2.8 mg) were implanted in a pouch created between the right oblique abdominal muscles in each animal. In Group A, the demineralized bone was implanted without prior sterilization of the material, whereas the demineralized bone implanted in Groups B, C, and D had been sterilized in ethylene oxide gas for 1, 3, or 6 hours, respectively, and aerated for 48 hours. At 4 weeks postoperatively, bone formation was evaluated quantitatively by strontium 85 uptake and qualitatively by light microscopy of histological sections. One-way analyses of variance at the 0.05 level revealed no significant difference in strontium 85 uptake of the different groups, and no qualitative differences in osteoinduction could be detected by light microscopy. Ossicles consisting of bone and bone marrow were seen in the recovered implants of all groups.
业已表明，脱矿骨的不同灭菌程序可能会影响其骨诱导特性。本研究的目的是评估环氧乙烷分别灭菌1小时、3小时和6小时对同种异体脱矿骨异位植入大鼠后的骨诱导潜能的影响。将60只雄性Wistar大鼠随机分为A至D四组，每组动物在右腹斜肌之间创建的袋囊中植入4片脱矿骨片（2.8毫克）。A组植入未经预先灭菌的脱矿骨，而B、C和D组植入的脱矿骨分别用环氧乙烷气体灭菌1小时、3小时或6小时，并通气48小时。术后4周，通过锶85摄取定量评估骨形成，并通过组织学切片的光学显微镜定性评估。在0.05水平进行的单因素方差分析显示，不同组的锶85摄取没有显著差异，光学显微镜也未检测到骨诱导的定性差异。所有组回收的植入物中均可见由骨和骨髓组成的小骨块。
