Arjmand Babak, Aghayan Hamid Reza, Larijani Bagher, Sahebjam Mehrnaz, Ghaderi Firoozeh, Goodarzi Parisa
Chronic Diseases Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran AND Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Acta Med Iran. 2014;52(3):215-9.
The gamma irradiation has been used for end sterilization of allograft bones and its effects with a 25 kGy dosage on the osteoinductive properties of demineralized bone allograft powder was studied. This work carried out using an experimental method in an animal model. In this study the demineralized bone allograft powder which had been sterilized and prepared with gamma irradiation in a 25 kGy dosage in 18 hours, was used as a study group and the demineralized bone allograft powder which had been prepared aseptically was used as the reference group. 30 mg of bone powder from each group were implanted into right and left paravertebral muscles of eighteen rats, separately. After four weeks, the implanted samples were harvested with a 0.5 cm border and then the osteoinductivity of implants in two groups were compared with histopathologic studies. In 94.4% of the reference samples a new bone formation was observed. In the study group, this difference was observed only in 27.7% of samples (P<0.002). It appears that using gamma irradiation may lead to a reduction in osteoinduction properties of demineralized bone allograft powder.
γ射线辐照已用于同种异体骨的终端灭菌，并研究了25千戈瑞剂量的γ射线辐照对脱矿骨同种异体骨粉骨诱导特性的影响。这项工作采用动物模型的实验方法进行。在本研究中，将在18小时内以25千戈瑞剂量进行γ射线辐照灭菌并制备的脱矿骨同种异体骨粉用作研究组，将无菌制备的脱矿骨同种异体骨粉用作参照组。将每组30毫克骨粉分别植入18只大鼠的左右椎旁肌。四周后，以0.5厘米的边界收获植入的样本，然后通过组织病理学研究比较两组植入物的骨诱导能力。在94.4%的参照样本中观察到新骨形成。在研究组中，仅在27.7%的样本中观察到这种差异（P<0.002）。看来使用γ射线辐照可能会导致脱矿骨同种异体骨粉的骨诱导特性降低。
