编码多形汉逊酵母中硝酸盐转运蛋白的YNT1基因与编码亚硝酸还原酶和硝酸还原酶的YNI1和YNR1基因聚集在一起，其缺失导致无法在硝酸盐中生长。
The YNT1 gene encoding the nitrate transporter in the yeast Hansenula polymorpha is clustered with genes YNI1 and YNR1 encoding nitrite reductase and nitrate reductase, and its disruption causes inability to grow in nitrate.
作者信息
Pérez M D, González C, Avila J, Brito N, Siverio J M
机构信息
Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, Tenerife, Canarias, Spain.
出版信息
Biochem J. 1997 Jan 15;321 ( Pt 2)(Pt 2):397-403. doi: 10.1042/bj3210397.
Abstract
DNA sequencing in the phage lambda JA13 isolated from a lambda EMBL3 Hansenula polymorpha genomic DNA library containing the nitrate reductase-(YNR1) and nitrite reductase-(YNI1) encoding genes revealed an open reading frame (YNT1) of 1524 nucleotides encoding a putative protein of 508 amino acids with great similarity to the nitrate transporters from Aspergillus nidulans and Chlamydomonas reinhardtii. Disruption of the chromosomal YNT1 copy resulted in incapacity to grow in nitrate and a significant reduction in rate of nitrate uptake. The disrupted strain is still sensitive to chlorate, and, in the presence of 0.1 mM nitrate, the expression of YNR1 and YNI1 and the activity of nitrate reductase and nitrite reductase are significantly reduced compared with the wild-type. Northern-blot analysis showed that YNT1 is expressed when the yeast is grown in nitrate and nitrite but not in ammonium solution.
摘要
从含有编码硝酸还原酶（YNR1）和亚硝酸还原酶（YNI1）基因的多形汉逊酵母λEMBL3基因组DNA文库中分离出的噬菌体λJA13的DNA测序显示，有一个1524个核苷酸的开放阅读框（YNT1），编码一个508个氨基酸的假定蛋白质，与构巢曲霉和莱茵衣藻的硝酸盐转运蛋白有高度相似性。染色体YNT1拷贝的破坏导致无法在硝酸盐中生长，硝酸盐摄取速率显著降低。被破坏的菌株对氯酸盐仍然敏感，并且在存在0.1 mM硝酸盐的情况下，与野生型相比，YNR1和YNI1的表达以及硝酸还原酶和亚硝酸还原酶的活性显著降低。Northern印迹分析表明，当酵母在硝酸盐和亚硝酸盐中生长时YNT1表达，但在铵溶液中不表达。
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The YNT1 gene encoding the nitrate transporter in the yeast Hansenula polymorpha is clustered with genes YNI1 and YNR1 encoding nitrite reductase and nitrate reductase, and its disruption causes inability to grow in nitrate.编码多形汉逊酵母中硝酸盐转运蛋白的YNT1基因与编码亚硝酸还原酶和硝酸还原酶的YNI1和YNR1基因聚集在一起，其缺失导致无法在硝酸盐中生长。
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