顺式不饱和脂肪酸对巨噬细胞肿瘤（AK-5）细胞体外细胞氧化应激的影响。

Effect of cis-unsaturated fatty acids on cellular oxidant stress in macrophage tumor (AK-5) cells in vitro.

作者信息

Padma M, Das U N

机构信息

Department of Medicine, Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad, India.

出版信息

Cancer Lett. 1996 Dec 3;109(1-2):63-75. doi: 10.1016/s0304-3835(96)04419-9.

DOI:10.1016/s0304-3835(96)04419-9

PMID:9020904

Abstract

Cis-unsaturated fatty acids (c-UFAs) induced decreased survival of macrophage tumor (AK-5) cells in vitro. The cytotoxic action of c-UfAs was associated with an increase in free radical generation and lipid peroxidation process. In addition, exposure of AK-5 cells to various c-UFAs for a short period (1 h) decreased the cellular concentrations of anti-oxidants: superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase, glutathione and vitamin E. However, prolonged (24 h) exposure of AK-5 cells to c-UFAs enhanced the levels of SOD with little or no change in the concentrations of catalase, glutathione peroxidase and glutathione reductase. These results indicate that c-UFAs can enhance free radical generation and lower the concentrations of various anti-oxidants in the tumor cells which may explain the cytotoxic action of c-UFAs.

摘要

顺式不饱和脂肪酸（c-UFAs）在体外可导致巨噬细胞肿瘤（AK-5）细胞存活率降低。c-UFAs的细胞毒性作用与自由基生成增加和脂质过氧化过程有关。此外，将AK-5细胞短期（1小时）暴露于各种c-UFAs会降低细胞内抗氧化剂的浓度，这些抗氧化剂包括超氧化物歧化酶（SOD）、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽和维生素E。然而，将AK-5细胞长期（24小时）暴露于c-UFAs会提高SOD的水平，而过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的浓度几乎没有变化或变化很小。这些结果表明，c-UFAs可增强肿瘤细胞中的自由基生成并降低各种抗氧化剂的浓度，这可能解释了c-UFAs的细胞毒性作用。