Durairaj Varalakshmi, Shakya Garima, Rajagopalan Rukkumani
Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry, India.
J Diet Suppl. 2015 Jun;12(2):126-37. doi: 10.3109/19390211.2014.902002. Epub 2014 Apr 3.
Alcohol abuse is recognized as the most common cause for the development of various abnormalities including liver disease. Excessive free radicals are generated during the metabolism of ethanol. The ingestion of alcohol along with polyunsaturated fatty acid (PUFA) aggravates the production of free radicals and enhances the oxidative stress. Medicinal plants contain active phytocomponents, which are the principal healthcare resources. We aimed to analyze the effect of wheatgrass extract on alcohol and thermally oxidized PUFA (ΔPUFA) induced oxidative stress in male albino Wistar rats. The levels of marker enzymes gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), lipid peroxidative markers; thiobarbutric acid reactive substances (TBARS) and lipid hydroperoxides (LH), the levels of enzymatic (catalase [CAT], superoxide dismutase [SOD], glutathione peroxidase [GPx]) and nonenzymatic (reduced glutathione [GSH], vitamin E, vitamin C) antioxidants were analyzed in liver to evaluate the effects of wheatgrass. The levels of TBARS and LH were significantly (p ≤ .05) increased in alcohol + ΔPUFA group, which were found to be reduced on treatment with wheatgrass. The levels of both enzymatic and nonenzymatic antioxidants were significantly (p ≤ .05) decreased in alcohol + ΔPUFA group, which were found to be restored on treatment with wheatgrass. From the results obtained, we conclude that wheatgrass protects the liver against alcohol and ΔPUFA induced oxidative stress.
酒精滥用被认为是包括肝脏疾病在内的各种异常情况发生的最常见原因。乙醇代谢过程中会产生过量自由基。酒精与多不饱和脂肪酸（PUFA）一同摄入会加剧自由基的产生并增强氧化应激。药用植物含有活性植物成分，这些是主要的医疗保健资源。我们旨在分析小麦草提取物对雄性白化Wistar大鼠中酒精和热氧化PUFA（ΔPUFA）诱导的氧化应激的影响。分析了肝脏中标记酶γ-谷氨酰转移酶（GGT）、碱性磷酸酶（ALP）、脂质过氧化标记物；硫代巴比妥酸反应性物质（TBARS）和脂质氢过氧化物（LH）的水平，以及酶促抗氧化剂（过氧化氢酶[CAT]、超氧化物歧化酶[SOD]、谷胱甘肽过氧化物酶[GPx]）和非酶促抗氧化剂（还原型谷胱甘肽[GSH]、维生素E、维生素C）的水平，以评估小麦草的作用。在酒精 + ΔPUFA组中，TBARS和LH的水平显著升高（p≤0.05），而在用小麦草治疗后发现这些水平降低。在酒精 + ΔPUFA组中，酶促和非酶促抗氧化剂的水平均显著降低（p≤0.05），而在用小麦草治疗后发现这些水平得以恢复。根据获得的结果，我们得出结论，小麦草可保护肝脏免受酒精和ΔPUFA诱导的氧化应激。
