将牛遗传图谱与物理图谱锚定的黏粒衍生标记。

Cosmid-derived markers anchoring the bovine genetic map to the physical map.

作者信息

Ferretti L, Urquhart B G, Eggen A, Olsaker I, Harlizius B, Castiglioni B, Mezzelani A, Solinas Toldo S, Thieven U, Zhang Y, Morgan A L, Teres V M, Schwerin M, Martin-Burriel I, Chowdhary B P, Erhardt G, Nijman I J, Cribiu E P, Barendse W, Leveziel H, Fries R, Williams J L

机构信息

IDVGA-CNR, Milan, Italy.

出版信息

Mamm Genome. 1997 Jan;8(1):29-36. doi: 10.1007/s003359900341.

DOI:10.1007/s003359900341

PMID:9021144

Abstract

The mapping strategy for the bovine genome described in this paper uses large insert clones as a tool for physical mapping and as a source of highly polymorphic microsatellites for genetic typing, and was one objective of the BovMap Project funded by the European Union (UE). Eight-three cosmid and phage clones were characterized and used to physically anchor the linkage groups defining all the bovine autosomes and the X Chromosome (Chr). By combining physical and genetic mapping, clones described in this paper have led to the identification of the linkage groups corresponding to Chr 9, 12, 16, and 25. In addition, anchored loci from this study were used to orient the linkage groups corresponding to Chr 3, 7, 8, 9, 13, 16, 18, 19, and 28 as identified in previously published maps. Comparison of the estimated size of the physical and linkage maps suggests that the genetic length of the bovine genome may be around 4000 cM.

摘要

本文所述的牛基因组图谱构建策略，使用大插入片段克隆作为物理图谱构建的工具以及用于基因分型的高度多态微卫星的来源，这是由欧盟资助的BovMap项目的一个目标。对83个黏粒和噬菌体克隆进行了表征，并用于物理定位定义所有牛常染色体和X染色体（Chr）的连锁群。通过结合物理图谱和遗传图谱，本文所述的克隆已导致鉴定出与Chr 9、12、16和25相对应的连锁群。此外，本研究中定位的位点被用于确定先前发表的图谱中与Chr 3、7、8、9、13、16、18、19和28相对应的连锁群的方向。物理图谱和连锁图谱估计大小的比较表明，牛基因组的遗传长度可能约为4000 cM。