Andoh Y, Kuramoto T, Yokoi N, Maihara T, Kitada K, Serikawa T
Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Japan.
Mamm Genome. 1998 Apr;9(4):287-93. doi: 10.1007/s003359900750.
To correlate rat genetic linkage maps with cytogenetic maps, we localized 25 new cosmid-derived simple sequence length polymorphism (SSLP) markers and 14 existing genetic markers on cytogenetic bands of chromosomes, using fluorescence in situ hybridization (FISH). Next, a total of 58 anchor loci, consisting of the 39 new and 19 previously reported ones, were integrated into the genetic linkage maps. Since most of the new anchor loci were developed to be localized near the terminals of the genetic or cytogenetic maps for each chromosome, the orientation and coverage of the whole genetic linkage maps were determined or confirmed with respect to the cytogenetic maps. Thus, we provide here a new base for rat genetic maps.
为了将大鼠遗传连锁图谱与细胞遗传图谱相关联，我们利用荧光原位杂交（FISH）技术，将25个新的黏粒衍生简单序列长度多态性（SSLP）标记和14个现有的遗传标记定位到染色体的细胞遗传带上。接下来，总共58个锚定基因座，包括39个新的和19个先前报道的基因座，被整合到遗传连锁图谱中。由于大多数新的锚定基因座是为定位在每条染色体的遗传或细胞遗传图谱末端附近而开发的，因此整个遗传连锁图谱的方向和覆盖范围得以相对于细胞遗传图谱确定或确认。因此，我们在此为大鼠遗传图谱提供了一个新的基础。
