Lau T M, Witjaksono J, Affandi B, Rogers P A
Department of Obstetrics and Gynaecology, Monash University, Monash Medical Centre, Clayton, Victoria, Australia.
Hum Reprod. 1996 Dec;11(12):2629-34. doi: 10.1093/oxfordjournals.humrep.a019183.
The expression of endometrial progesterone receptor mRNA during the human menstrual cycle and in Norplant users was studied using digoxigenin-labelled ribonucleic probes for in-situ hybridization on 6 microns paraffin embedded endometrial sections. The staining intensity was scored blind semi-quantitatively. Blood ovarian steroid concentrations were measured in Norplant users. All data were analysed by analysis of variance. Glandular progesterone receptor mRNA concentrations were low during the menstrual-to-early proliferative stage but increased during the early-to-mid to late-proliferative stage then declined non-significantly over the secretory stage. No such variation was observed in stromal cells. Progesterone receptor mRNA concentrations were lower in Norplant than controls during early-to-mid to late-proliferative stages (in glandular epithelium and stroma) and during secretory stage (in stroma only). Norplant subjects with amenorrhoea had higher concentrations of stromal progesterone receptor mRNA but lower plasma oestrogen concentrations than subjects with breakthrough bleeding. The pattern of variation in progesterone receptor mRNA concentrations during the normal menstrual cycle resembles the published pattern for the receptor protein. The results demonstrate: (i) a differential sensitivity of glandular and stromal progesterone receptors to steroid regulation; (ii) in contrast to previous findings of an increase in immunoreactive progesterone receptor protein in Norplant endometrium, progesterone receptor mRNA concentrations in these tissues were reduced; and (iii) there was significantly more progesterone receptor mRNA in subjects with amenorrhoea than in those with breakthrough bleeding.
采用地高辛标记的核糖核酸探针，对6微米石蜡包埋的子宫内膜切片进行原位杂交，研究了人月经周期及使用诺普兰（Norplant）的妇女子宫内膜孕酮受体mRNA的表达情况。对染色强度进行盲法半定量评分。测定了使用诺普兰的妇女血中卵巢甾体激素浓度。所有数据均采用方差分析。在月经至增殖早期，腺上皮孕酮受体mRNA浓度较低，但在增殖早期至中期以及晚期有所升高，然后在分泌期无显著下降。间质细胞未观察到这种变化。在增殖早期至中期以及晚期（腺上皮和间质）和分泌期（仅间质），使用诺普兰的妇女孕酮受体mRNA浓度低于对照组。闭经的诺普兰使用者间质孕酮受体mRNA浓度较高，但血浆雌激素浓度低于突破性出血者。正常月经周期中孕酮受体mRNA浓度的变化模式与已发表的受体蛋白模式相似。结果表明：（i）腺上皮和间质孕酮受体对甾体激素调节的敏感性不同；（ii）与先前关于诺普兰使用者子宫内膜免疫反应性孕酮受体蛋白增加的研究结果相反，这些组织中孕酮受体mRNA浓度降低；（iii）闭经者的孕酮受体mRNA明显多于突破性出血者。
