Clement-Sengewald A, Schütze K, Ashkin A, Palma G A, Kerlen G, Brem G
Bayerische Klonierungsforschungs-GmbH & Co. KG, Oberschleissheim, Germany.
J Assist Reprod Genet. 1996 Mar;13(3):259-65. doi: 10.1007/BF02065947.
Our purpose was to show that fertilization of oocytes can be obtained solely by laser light-mediated manipulation of gametes.
A small channel was drilled into the zona pellucida of bovine oocytes using an ultraviolet (UV)-laser microbeam. Highly diluted cattle sperm were not able to fertilize the laser drilled oocytes.
Fertilization was achieved only when three to five cattle sperm were trapped with optical tweezers and inserted directly through the laser drilled hole into the perivitelline space. After 20 hr, 3 of 79 (3.8%) oocytes revealed two pronuclei and a sperm tail within their cytoplasm. Cattle sperm are difficult to catch. Therefore, the gametes had to remain for about 20 min in room atmosphere, which might be the reason for the low fertilization results.
The results indicate that a combined UV-laser microbeam and optical tweezers trap can be used successfully for "noncontact" microinsemination procedures.
我们的目的是证明仅通过激光介导的配子操作就可以实现卵母细胞的受精。
使用紫外（UV）激光微束在牛卵母细胞的透明带上钻一个小通道。高度稀释的牛精子无法使经激光打孔的卵母细胞受精。
只有当用光学镊子捕获三到五个牛精子并直接通过激光打孔插入卵周隙时才能实现受精。20小时后，79个卵母细胞中有3个（3.8%）在其细胞质内显示出两个原核和一条精子尾巴。牛精子很难捕获。因此，配子必须在室内空气中停留约20分钟，这可能是受精率低的原因。
结果表明，紫外激光微束和光学镊子捕获相结合可成功用于“非接触”显微授精程序。
