Alegría A, Barberá R, Clemente G, Farré R, García M J, Lagarda M J
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia Spain.
J Trace Elem Med Biol. 1996 Dec;10(4):223-8.
In order to assess the reference values for selenium nutritional status, adequate indicators (selenium concentration and glutathione peroxidase activity) were determined in whole blood and blood derivates of a healthy population (n = 287) from the province of Valencia, Spain. The reference population was selected by applying preestablished criteria. Selenium in whole blood and plasma was measured by graphite furnace atomic absorption spectrometry (GFAAS), with a deuterium correction, after addition of Pd/Mg(NO3)2 as the matrix modifier and appropriate dilution. Accuracy was checked by means of a reference material (Seronorm Trace Metals serum and whole blood). The population's reference intervals for selenium content at a 95% confidence level were: 53.03-108.96 and 66.71-119.4 mg/L for plasma and whole blood selenium concentration respectively. GPX activity was measured using a modification of the Paglia and Valentine method, and the reference intervals obtained ranged from 196 to 477 U/L in plasma, from 49 to 93 U/gHb in erythrocytes and from 52 to 96 U/gHb in whole blood. The only statistically significant differences detected between men and women are for to the GPX activity in whole blood. The results obtained are in the range of values found by others authors in healthy populations residing in different European countries.
为了评估硒营养状况的参考值，我们测定了西班牙巴伦西亚省健康人群（n = 287）全血及血液衍生物中的适当指标（硒浓度和谷胱甘肽过氧化物酶活性）。参考人群通过应用预先制定的标准进行选择。全血和血浆中的硒采用石墨炉原子吸收光谱法（GFAAS）测定，添加Pd/Mg(NO3)2作为基体改进剂并进行适当稀释后，采用氘校正。通过参考物质（Seronorm痕量金属血清和全血）检查准确性。该人群在95%置信水平下的硒含量参考区间分别为：血浆硒浓度为53.03 - 108.96 mg/L，全血硒浓度为66.71 - 119.4 mg/L。GPX活性采用对Paglia和Valentine方法的改进进行测定，得到的参考区间为：血浆中196至477 U/L，红细胞中49至93 U/gHb，全血中52至96 U/gHb。男性和女性之间检测到的唯一具有统计学意义的差异是全血中的GPX活性。所得结果在其他作者对居住在不同欧洲国家的健康人群所发现的值的范围内。
