van Leeuwen I S, Cornelisse C J, Misdorp W, Goedegebuure S A, Kirpensteijn J, Rutteman G R
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Cancer Lett. 1997 Jan 1;111(1-2):173-8. doi: 10.1016/s0304-3835(96)04529-6.
Osteosarcomas in 18 dogs were examined for the presence of p53 mutations in exons 4-8 by single strand conformation polymorphism (SSCP) analysis, followed by sequence analysis in tumors demonstrating abnormal bands in the SSCP analysis. P53 mutations were found in four of the primary tumors in 17 dogs. Metastases studied in two of these dogs in which the primary tumor contained only wild type p53 did not contain mutations, nor those of one dog in which the primary tumor was not studied. The alterations that were found included three missense mutations and one 3 bp insertion.
通过单链构象多态性（SSCP）分析，检测了18只犬骨肉瘤中外显子4 - 8的p53突变情况，随后对SSCP分析显示有异常条带的肿瘤进行测序分析。在17只犬的4个原发性肿瘤中发现了p53突变。在其中2只原发性肿瘤仅含野生型p53的犬中研究的转移灶未发现突变，在1只未对原发性肿瘤进行研究的犬中转移灶也未发现突变。发现的改变包括3个错义突变和1个3 bp插入突变。
