Razumas V, Talaikyte Z, Barauskas J, Larsson K, Miezis Y, Nylander T
Department of Bioelectrochemistry, Institute of Biochemistry, Vilnius, Lithuania.
Chem Phys Lipids. 1996 Dec 20;84(2):123-88. doi: 10.1016/s0009-3084(96)02629-1.
X-ray diffraction and Raman scattering spectroscopy have been used to study phase transitions and changes in molecular organization of the cubic Pn3m monoolein (MO)-H2O phase upon introducing low amounts of distearoylphosphatidylglycerol (DSPG) and lysozyme (LSZ). X-ray diffraction measurements indicated a phase transition Pn3m-Im3m brought about by DSPG and LSZ, however DSPG also induced formation of the lamellar phase. Raman spectra have demonstrated that incorporation of DSPG into the lipid bilayer decreases the mobility of acyl chains and increases the number of hydrogen-bonded C=O groups of MO. On the other hand, LSZ exerts identical effect on the latter parameter, while no effect on the state of acyl chains order was observed. This result and differential scanning calorimetric measurements indicate that LSZ is located in the water channel system of the cubic phase. The results are discussed on the basis of an infinite periodical lipid bilayer structure and lipid parameter concepts.
利用X射线衍射和拉曼散射光谱研究了在引入少量二硬脂酰磷脂酰甘油（DSPG）和溶菌酶（LSZ）后，立方相Pn3m单油酸甘油酯（MO）-H₂O相的相变和分子排列变化。X射线衍射测量表明，DSPG和LSZ引起了Pn3m-Im3m相变，然而DSPG也诱导了层状相的形成。拉曼光谱表明，将DSPG掺入脂质双层会降低酰基链的流动性，并增加MO中氢键结合的C=O基团的数量。另一方面，LSZ对后一个参数有相同的影响，而未观察到对酰基链有序状态的影响。这一结果和差示扫描量热测量表明，LSZ位于立方相的水通道系统中。基于无限周期性脂质双层结构和脂质参数概念对结果进行了讨论。
