Nakahara K, Saito H, Saito T, Ito M, Ohta N, Sakai N, Tezuka N, Hiroi M, Watanabe H
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Japan.
Fertil Steril. 1997 Feb;67(2):302-8. doi: 10.1016/S0015-0282(97)81915-2.
To investigate the incidence of apoptotic bodies in mural granulosa cell masses and cumulus cell masses.
Nonrandomized, prospective study.
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, Japan.
PATIENT(S): One hundred twenty-nine normally ovulating women underwent ovulation induction for IVF-ET with GnRH analogue (GnRH-a) and gonadotropins.
INTERVENTION(S): Patients underwent follicle aspiration after the administration of hCG.
MAIN OUTCOME MEASURE(S): The nuclei of recovered granulosa cells were examined by fluorescence microscopy and the incidence of apoptotic bodies was tabulated.
RESULT(S): The incidence of apoptotic bodies was significantly higher in mural granulosa cell masses than in cumulus cell masses in the entire group of 129 patients. Both incidence of apoptotic bodies of mural granulosa cell masses and cumulus cell masses were significantly higher in patients with less than six follicular oocytes compared with patients with six or more oocytes. Nonpregnant patients showed significantly higher incidence of apoptotic bodies in mural granulosa cell masses compared with pregnant patients.
CONCLUSION(S): These results indicate that mural granulosa cell masses and cumulus cell masses may have different functions in follicular maturation. The incidence of apoptotic bodies in mural granulosa cell masses can be used as an indicator of success of IVF.
研究壁颗粒细胞团和卵丘细胞团中凋亡小体的发生率。
非随机前瞻性研究。
日本山形县山形大学医学院妇产科。
129名正常排卵女性接受促性腺激素释放激素类似物（GnRH-a）和促性腺激素诱导排卵以进行体外受精-胚胎移植（IVF-ET）。
患者在注射人绒毛膜促性腺激素（hCG）后进行卵泡抽吸。
通过荧光显微镜检查回收的颗粒细胞的细胞核，并将凋亡小体的发生率制成表格。
在129名患者的整个队列中，壁颗粒细胞团中凋亡小体的发生率显著高于卵丘细胞团。与有6个或更多卵泡卵母细胞的患者相比，卵泡卵母细胞少于6个的患者壁颗粒细胞团和卵丘细胞团中凋亡小体的发生率均显著更高。与妊娠患者相比，未妊娠患者壁颗粒细胞团中凋亡小体的发生率显著更高。
这些结果表明壁颗粒细胞团和卵丘细胞团在卵泡成熟过程中可能具有不同的功能。壁颗粒细胞团中凋亡小体的发生率可作为IVF成功的指标。
