Nakahara K, Saito H, Saito T, Ito M, Ohta N, Takahashi T, Hiroi M
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Japan.
Fertil Steril. 1997 Aug;68(2):312-7. doi: 10.1016/s0015-0282(97)81521-x.
To investigate the relationship between the incidence of apoptotic bodies in membrana granulosa and follicular steroid concentrations in human follicles.
Case-controlled prospective study for 132 individual follicles.
Procedures were performed in Yamagata University Hospital.
PATIENT(S): Thirty-six normo-ovulatory women with tubal infertility underwent ovulation induction for IVF-ET with a conventional hyperstimulation method.
INTERVENTION(S): Patients underwent follicle aspiration after the administration of hCG.
MAIN OUTCOME MEASURE(S): The nuclei of recovered granulosa cells were examined by fluorescence microscopy, and the incidence of apoptotic bodies was tabulated. Intrafollicular steroids were evaluated mainly by RIA. These data were analyzed with respect to oocyte-retrieval, oocyte maturity, fertilization, and embryo quality.
RESULT(S): Membrana granulosa cells in the follicles from which oocytes were subsequently fertilized showed a significantly lower incidence of apoptotic bodies than those in follicles from which the oocytes did not fertilize. Membrana granulosa cells in the follicles from which oocytes were developed into good quality showed a significantly lower incidence of apoptotic bodies than those in the follicles from which oocytes developed into fair and poor quality. The incidence of apoptotic bodies was significantly higher in the mural granulosa cell region than in the cumulus cell region in most cases. Intrafollicular E2, P, and free T levels were not different between the oocyte groups.
CONCLUSION(S): These results indicate that lower incidence of apoptotic bodies in individual follicles is associated with better outcomes for oocytes. Also, mural granulosa cells and cumulus cell in each follicle may show differentiation during follicular maturation.
研究人类卵泡颗粒膜中凋亡小体的发生率与卵泡类固醇浓度之间的关系。
对132个单个卵泡进行病例对照前瞻性研究。
在山形大学医院进行相关操作。
36名输卵管性不孕的排卵正常女性，采用传统超刺激方法进行体外受精 - 胚胎移植的促排卵治疗。
患者在注射人绒毛膜促性腺激素后进行卵泡抽吸。
通过荧光显微镜检查回收的颗粒细胞的细胞核，并将凋亡小体的发生率制成表格。卵泡内类固醇主要通过放射免疫分析法进行评估。这些数据针对取卵、卵母细胞成熟度、受精情况和胚胎质量进行分析。
随后其卵母细胞受精的卵泡中的颗粒膜细胞凋亡小体的发生率显著低于卵母细胞未受精的卵泡中的颗粒膜细胞。卵母细胞发育为优质胚胎的卵泡中的颗粒膜细胞凋亡小体的发生率显著低于卵母细胞发育为中等质量和低质量胚胎的卵泡中的颗粒膜细胞。在大多数情况下，壁层颗粒细胞区域凋亡小体的发生率显著高于卵丘细胞区域。卵母细胞组之间卵泡内的雌二醇、孕酮和游离睾酮水平没有差异。
这些结果表明，单个卵泡中凋亡小体发生率较低与卵母细胞的更好结局相关。此外，每个卵泡中的壁层颗粒细胞和卵丘细胞在卵泡成熟过程中可能会表现出分化。
