Benoff S, Hurley I R, Barcia M, Mandel F S, Cooper G W, Hershlag A
North Shore University Hospital, Boas-Marks Biomedical Science Research Center, New York 11030, USA.
Fertil Steril. 1997 Feb;67(2):336-47. doi: 10.1016/S0015-0282(97)81921-8.
To determine whether mannose ligand receptor and acrosome reaction deficits in sperm from men with varicocele are related to the transition metal content of their semen.
Cadmium and zinc in semen and blood plasma were assayed for fertile males, men without varicocele who required intracytoplasmic sperm injection to achieve fertilization, and men evaluated for potential varicocele-associated infertility. The relationship between actin cytoskeletal distributions and acrosome status was determined for fertile donor sperm in the presence and absence of exogenous cadmium.
University hospital-based molecular biology research laboratory.
PATIENT(S): Patients from two university hospital-based IVF-assisted reproductive technology programs and two male urology private practices.
INTERVENTION(S): Fertile donor sperm were exposed to exogenous cadmium during capacitating incubations followed by culture at temperatures up to 41 degrees C.
MAIN OUTCOME MEASURE(S): Metal ion levels in semen and blood plasma were determined by graphite furnace atomic absorption spectroscopy. Motile sperm were examined for mannose ligand binding and the ability to undergo spontaneous and induced acrosome reactions. Unfixed, Triton-permeabilized sperm were probed with antiactin and antimyosin antibodies.
RESULT(S): Cadmium was elevated and zinc was decreased in the seminal plasma of men with varicocele. The content of these metals in semen and blood was not correlated. Cadmium exposure in vitro reduced mannose receptor expression, acrosome exocytosis, and cytoskeletal formation by fertile donor sperm.
CONCLUSION(S): Defects in transition metal regulation or excessive cadmium exposure are involved in varicocele-associated infertility.
确定精索静脉曲张男性精子中的甘露糖配体受体和顶体反应缺陷是否与其精液中的过渡金属含量有关。
对生育能力正常的男性、需要通过卵胞浆内单精子注射实现受精的无精索静脉曲张男性以及评估有潜在精索静脉曲张相关性不育的男性的精液和血浆中的镉和锌进行测定。在有和没有外源性镉存在的情况下，确定生育能力正常的供体精子中肌动蛋白细胞骨架分布与顶体状态之间的关系。
大学医院的分子生物学研究实验室。
来自两个大学医院的体外受精辅助生殖技术项目以及两个男性泌尿外科私人诊所的患者。
在获能培养期间，将生育能力正常的供体精子暴露于外源性镉，然后在高达41摄氏度的温度下培养。
通过石墨炉原子吸收光谱法测定精液和血浆中的金属离子水平。检查活动精子的甘露糖配体结合能力以及自发和诱导顶体反应的能力。用抗肌动蛋白和抗肌球蛋白抗体检测未固定、经曲拉通通透处理的精子。
精索静脉曲张男性的精浆中镉含量升高而锌含量降低。精液和血液中这些金属的含量不相关。体外镉暴露降低了生育能力正常的供体精子的甘露糖受体表达、顶体胞吐作用和细胞骨架形成。
过渡金属调节缺陷或镉暴露过多与精索静脉曲张相关性不育有关。
