Das N N, Das A
Carbohydr Res. 1977 Jul;56(2):337-49. doi: 10.1016/s0008-6215(00)83354-6.
Hot-water extraction of defatted garlic-bulbs yielded a mixture of polysaccharides containing a D-galactan, a D-galacturonan, an L-arabinan, a D-glucan, and a D-fructan. A trace of L-rhamnose was also detected in the polysaccharide hydrolyzate. The pectic acid was partially removed by precipitation with aqueous calcium chloride; from the remaining polysaccharide mixture, a pure D-galactan containing 97.3% of D-galactose was isolated by fractional precipitation and repeated chromatography through a column of DEAE-cellulose. Methanolysis and hydrolysis of the permethylated D-galactan yielded 2,3,4,6-tetra-, 2,3,6-tri-, and 2,3,di-O-methyl-D-galactose in the molar proportions of 1:2:1. On periodate oxidation, the D-galactan reduced 1.18 molar equivalents of the oxidant per D-galactosyl residue, and liberated one molar equivalent of formic acid per 4.13 D-galactosyl residues. Smith degradation of the D-galactan was also conducted. From these results, a structure has been assigned to the repeating unit of the D-galactan.
对脱脂蒜球进行热水提取，得到了一种多糖混合物，其中含有D-半乳聚糖、D-半乳糖醛酸聚糖、L-阿拉伯聚糖、D-葡聚糖和D-果聚糖。在多糖水解产物中还检测到微量的L-鼠李糖。通过用氯化钙水溶液沉淀法部分去除果胶酸；从剩余的多糖混合物中，通过分级沉淀和通过DEAE-纤维素柱的反复色谱法分离出一种纯度为97.3%的纯D-半乳聚糖，其含有D-半乳糖。全甲基化D-半乳聚糖的甲醇解和水解产生了摩尔比例为1:2:1的2,3,4,6-四-O-甲基-D-半乳糖、2,3,6-三-O-甲基-D-半乳糖和2,3-二-O-甲基-D-半乳糖。在高碘酸盐氧化过程中，D-半乳聚糖每个D-半乳糖基残基还原1.18摩尔当量的氧化剂，每4.13个D-半乳糖基残基释放1摩尔当量的甲酸。还对D-半乳聚糖进行了史密斯降解。根据这些结果，确定了D-半乳聚糖重复单元的结构。
