Wolff K, Garretty D, Hay A W
School of Medicine, Division of Clinical Sciences, University of Leeds, Old Medical School, UK.
Ann Clin Biochem. 1997 Jan;34 ( Pt 1):61-7. doi: 10.1177/000456329703400109.
We have developed a micro-extraction procedure for the analysis of seven commonly prescribed benzodiazepines (chlordiazepoxide, diazepam, lorazepam, nitrazepam, nordiazepam, oxazepam, and temazepam) in urine using liquid chromatography. The method is reliable and sensitive, uses small volumes (100 microL) of urine and is suitable for the detection and quantification of low concentrations of benzodiazepines. The micro-extraction procedure allowed rapid sample processing, which is important for routine sample handling. The limit of detection for the seven benzodiazepines ranged from 0.10-0.71 mg/L and recovery of the different benzodiazepines was good, ranging from 70-105%. Between- and within-assay coefficients of variation ranged from 6.3% to 13.8%, and 2% to 3.5%, respectively. Chlordiazepoxide chromatographed poorly (between assay coefficient of variation 35.4%, within-assay 7%), and we set the cut-off value for this compound at 5.0 mg/L.
我们开发了一种微萃取方法，用于采用液相色谱法分析尿液中七种常用的苯二氮䓬类药物（氯氮卓、地西泮、劳拉西泮、硝西泮、去甲西泮、奥沙西泮和替马西泮）。该方法可靠且灵敏，使用少量（100微升）尿液，适用于检测和定量低浓度的苯二氮䓬类药物。微萃取方法可实现快速样品处理，这对于常规样品处理很重要。七种苯二氮䓬类药物的检测限为0.10 - 0.71毫克/升，不同苯二氮䓬类药物的回收率良好，为70% - 105%。批间和批内变异系数分别为6.3%至13.8%和2%至3.5%。氯氮卓的色谱分离效果较差（批间变异系数35.4%，批内7%），我们将该化合物的临界值设定为5.0毫克/升。
