Garretty D J, Wolff K, Hay A W
Department of Chemical Pathology and Immunology, General Infirmary at Leeds, Old Medical School.
Ann Clin Biochem. 1998 Jul;35 ( Pt 4):528-33. doi: 10.1177/000456329803500407.
We have developed a micro-extraction procedure for the analysis of chlordiazepoxide and its two unique metabolites, demoxepam and desmethylchlordiazepoxide, in plasma, using liquid chromatography. The method is both reliable and sensitive for the quantitation of low plasma concentrations of these three compounds. The extraction procedure allows rapid sample processing, which, together with the small sample volume (100 microL), makes it ideal for routine sample handling. The limit of detection for the three compounds ranged from 0.075 to 0.125 mg/L and recovery of the three different benzodiazepines ranged from 87 to 94%. Within- and between-assay coefficients of variation ranged from 3.1-4.5% and from 4.7 to 7.6%, respectively.
我们开发了一种微萃取方法，用于使用液相色谱法分析血浆中的氯氮卓及其两种独特代谢物地莫西泮和去甲氯氮卓。该方法对于定量测定这三种化合物的低血浆浓度既可靠又灵敏。萃取程序允许快速处理样品，再加上小样本体积（100微升），使其成为常规样品处理的理想选择。这三种化合物的检测限范围为0.075至0.125毫克/升，三种不同苯二氮卓类药物的回收率范围为87%至94%。批内和批间变异系数分别为3.1-4.5%和4.7至7.6%。
