Zhou R, Zaki A E, Eisenmann D R
Department of Oral Biology, University of Illinois at Chicago 60612, USA.
Arch Oral Biol. 1996 Aug-Sep;41(8-9):739-47. doi: 10.1016/s0003-9969(96)00078-7.
Female Sprague-Dawley rats had 6 weeks of 0 (control), 75 or 100 parts/10(6) sodium fluoride in their drinking water. Whole mandibular incisors were removed, fixed, demineralized and sections prepared for light-microscopic morphometric analysis of dose-related alterations in enamel protein retention. Other rats given 0 and 75 parts/10(6) only (control and experimental groups) were used for autoradiographic evaluation of alterations in enamel protein removal 35S-methionine was applied directly over secretory ameloblasts at the end of the fifth week of fluoride exposure. Incisors were removed either 5 or 7 days later and processed for autoradiographic analysis. The results indicated: (1) extended retention of enamel proteins in fluoride-exposed maturation enamel as well as reduced enamel protein synthesis and/or secretion in the secretory stage; (2) negative linear correlation between extended enamel protein retention and reduced enamel protein secretion among groups; and (3) repression of enamel protein removal. The data are also consistent with the concept that the fluoride effect is multifactorial.
雌性斯普拉格-道利大鼠饮用含0（对照）、75或100 ppm氟化钠的水6周。取下整个下颌切牙，固定、脱矿，并制备切片用于光镜形态计量分析，以研究牙釉质蛋白保留的剂量相关变化。仅给予0和75 ppm氟化钠的其他大鼠（对照组和实验组）用于放射自显影评估牙釉质蛋白去除的变化。在氟暴露第5周结束时，将35S-甲硫氨酸直接应用于分泌期成釉细胞上。5天或7天后取下切牙并进行放射自显影分析。结果表明：（1）在氟暴露的成熟牙釉质中牙釉质蛋白保留时间延长，以及在分泌期牙釉质蛋白合成和/或分泌减少；（2）各实验组之间牙釉质蛋白保留时间延长与牙釉质蛋白分泌减少呈负线性相关；（3）牙釉质蛋白去除受到抑制。这些数据也与氟的作用是多因素的这一概念一致。
