Taran F, Frobert Y, Créminon C, Grassi J, Olichon D, Mioskowski C, Pradelles P
CEA, Service des Molécules Marquées DBCM, Gif sur Yvette, France.
Clin Chem. 1997 Feb;43(2):363-8.
A fast competitive enzyme immunoassay (EIA) for measuring homovanillic acid in human urine samples was developed with a monoclonal antibody and acetylcholinesterase as enzyme label. Enzyme detection was performed by an easy colorimetric assay. Monoclonal antibodies were screened on the basis of sensitivity, specificity, and correlation studies. EIA has a detection limit of 0.5 micromol/L, a CV <10% in the 1.25-10 micromol/L range, and intra- and interassay CVs of <10%. Cross-reactivity with vanillylmandelic acid was 0.5% and <8% for other structurally related catecholamine metabolites. Parallelism of the EIA was shown in dilution studies and the correlation with routine HPLC assay in 62 normal and pathologic samples was EIA = 1.492 (HPLC) - 3.46, S(y/x), = 47.52, range = 4-1800 micromol/L, r2 = 0.977. Additional data concerning the validity of this assay were provided by HPLC analysis of urinary immunoreactive material.
利用单克隆抗体和乙酰胆碱酯酶作为酶标记物，开发了一种用于检测人尿样中高香草酸的快速竞争性酶免疫分析（EIA）方法。通过简便的比色法进行酶检测。基于敏感性、特异性和相关性研究筛选单克隆抗体。EIA的检测限为0.5微摩尔/升，在1.25 - 10微摩尔/升范围内变异系数（CV）<10%，批内和批间CV均<10%。与香草扁桃酸的交叉反应率为0.5%，与其他结构相关的儿茶酚胺代谢产物的交叉反应率<8%。在稀释研究中显示了EIA的平行性，在62份正常和病理样本中，EIA与常规高效液相色谱（HPLC）分析的相关性为EIA = 1.492（HPLC） - 3.46，标准误S(y/x) = 47.52，范围 = 4 - 1800微摩尔/升，r2 = 0.977。通过对尿中免疫反应性物质的HPLC分析提供了有关该分析方法有效性的其他数据。
