Taran F, Bernard H, Valleix A, Créminon C, Grassi J, Olichon D, Deverre J R, Pradelles P
CEA, Service des Molécules Marquées DBCM, CEA-Saclay, Gif sur Yvette, France.
Clin Chim Acta. 1997 Aug 29;264(2):177-92. doi: 10.1016/s0009-8981(97)00092-2.
An enzyme immunoassay for urinary vanillylmandelic acid (VMA) using polyclonal antiserum and VMA-acetylcholinesterase conjugate as enzymatic tracer is described. Two different strategies for immunogen preparation were developed and enantioselectivity was demonstrated. Selected EIA allowed direct measurement of urinary VMA using D(-)-VMA as standard with good sensitivity (MDC = 0.1 mumol/l) and precision (CV less than 7% in 0.2-2.25 mumol/l range). Cross-reactivity with homovanillic acid (HVA) was 0.8% and less than 0.4% with other structurally related catecholamine metabolites. Intra- and inter-assay repeatability were less than 10% and recovery was 97.3% +/- 3%. Good correlation was obtained for EIA and HPLC analysis with normal and pathologic human urine samples (EIA = 0.895 HPLC-7.085, r2 = 0.98, n = 47).
本文描述了一种使用多克隆抗血清和VMA-乙酰胆碱酯酶偶联物作为酶标记物的尿香草扁桃酸（VMA）酶免疫测定法。开发了两种不同的免疫原制备策略，并证明了对映体选择性。选定的酶免疫测定法允许以D（-）-VMA作为标准直接测量尿VMA，具有良好的灵敏度（检测限=0.1μmol/L）和精密度（在0.2-2.25μmol/L范围内变异系数小于7%）。与高香草酸（HVA）的交叉反应率为0.8%，与其他结构相关的儿茶酚胺代谢物的交叉反应率小于0.4%。批内和批间重复性均小于10%，回收率为97.3%±3%。酶免疫测定法与高效液相色谱法对正常和病理人类尿液样本的分析具有良好的相关性（酶免疫测定法=0.895高效液相色谱法-7.085，r2=0.98，n=47）。
