枯草芽孢杆菌芽孢形成基因spoIIID的信使核糖核酸（mRNA）的翻译依赖于一个小的上游开放阅读框的翻译。
Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame.
作者信息
Decatur A, McMurry M T, Kunkel B N, Losick R
机构信息
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
出版信息
J Bacteriol. 1997 Feb;179(4):1324-8. doi: 10.1128/jb.179.4.1324-1328.1997.
Abstract
We report the existence of a small open reading frame (usd) that is located between the promoter and coding sequence for the sporulation gene spoIIID in Bacillus subtilis. The mRNA from the usd-spoIIID operon contains an inverted repeat sequence that is predicted to form a stem-loop structure that would sequester the ribosome binding site for spoIIID. A mutation eliminating the ribosome binding site for the upstream open reading frame caused an oligosporogenous phenotype and interfered with the translation, but not the transcription, of the downstream gene spoIIID. We propose that efficient synthesis of SpoIIID requires that the putative stem-loop structure be disrupted by translation through the upstream open reading frame.
摘要
我们报道了在枯草芽孢杆菌中，一个小的开放阅读框（usd）的存在，它位于芽孢形成基因spoIIID的启动子和编码序列之间。来自usd-spoIIID操纵子的mRNA包含一个反向重复序列，预计该序列会形成一个茎环结构，从而隔离spoIIID的核糖体结合位点。消除上游开放阅读框的核糖体结合位点的突变导致了少孢表型，并干扰了下游基因spoIIID的翻译，但不影响其转录。我们提出，SpoIIID的有效合成要求假定的茎环结构被上游开放阅读框的翻译所破坏。
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