组氨酸243在大肠杆菌EnvZ磷酸酶活性中的作用。
Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.
作者信息
Skarphol K, Waukau J, Forst S A
机构信息
Department of Biological Sciences, University of Wisconsin--Milwaukee, 53201, USA.
出版信息
J Bacteriol. 1997 Feb;179(4):1413-6. doi: 10.1128/jb.179.4.1413-1416.1997.
Abstract
EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH243Y were both shown to possess phosphatase activity in vitro. In addition, the mutant proteins were able to reduce the high level of OmpR-phosphate present in the envZ473 strain. These results indicate that His243 of EnvZ is not essential for stimulating the dephosphorylation of OmpR-phosphate.
摘要
EnvZ在His243处发生自身磷酸化，随后将磷酸基团转移至OmpR。EnvZ还具有OmpR - 磷酸磷酸酶活性。我们通过将His分别替换为Val、Tyr、Ser、Asp或Asn来研究His243在磷酸酶功能中的作用。EnvZH243V和EnvZH243Y在体外均显示具有磷酸酶活性。此外，突变蛋白能够降低envZ473菌株中高水平的OmpR - 磷酸。这些结果表明，EnvZ的His243对于刺激OmpR - 磷酸的去磷酸化并非必不可少。
相似文献
1
Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.组氨酸243在大肠杆菌EnvZ磷酸酶活性中的作用。
J Bacteriol. 1997 Feb;179(4):1413-6. doi: 10.1128/jb.179.4.1413-1416.1997.
2
Reverse phosphotransfer from OmpR to EnvZ in a kinase-/phosphatase+ mutant of EnvZ (EnvZ.N347D), a bifunctional signal transducer of Escherichia coli.在大肠杆菌双功能信号转导蛋白EnvZ的激酶功能缺失/磷酸酶功能增强突变体（EnvZ.N347D）中，OmpR向EnvZ的反向磷酸转移。
J Biol Chem. 1996 Jan 19;271(3):1424-9. doi: 10.1074/jbc.271.3.1424.
3
The critical role of the conserved Thr247 residue in the functioning of the osmosensor EnvZ, a histidine Kinase/Phosphatase, in Escherichia coli.保守的苏氨酸247残基在大肠杆菌中渗透压感受器EnvZ（一种组氨酸激酶/磷酸酶）功能发挥中的关键作用。
J Biol Chem. 2000 Dec 8;275(49):38645-53. doi: 10.1074/jbc.M005872200.
4
Interaction of EnvZ, a sensory histidine kinase, with phosphorylated OmpR, the cognate response regulator.感受态组氨酸激酶EnvZ与同源应答调节因子磷酸化OmpR的相互作用。
Mol Microbiol. 2002 Dec;46(5):1283-94. doi: 10.1046/j.1365-2958.2002.03240.x.
5
Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli.保守组氨酸-243在EnvZ磷酸酶活性中的作用，EnvZ是大肠杆菌中孔蛋白渗透调节的传感器。
J Bacteriol. 1997 Jun;179(11):3729-35. doi: 10.1128/jb.179.11.3729-3735.1997.
6
Molecular analysis of the signaling pathway between EnvZ and OmpR in Escherichia coli.大肠杆菌中EnvZ与OmpR之间信号通路的分子分析。
J Bacteriol. 1992 Mar;174(5):1522-7. doi: 10.1128/jb.174.5.1522-1527.1992.
7
Transmembrane signal transduction and osmoregulation in Escherichia coli: I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR.大肠杆菌中的跨膜信号转导与渗透调节：I. 对EnvZ和OmpR这两个调节成分之间磷酸转移所涉及的氨基酸残基进行定点诱变分析。
J Biochem. 1990 Sep;108(3):483-7. doi: 10.1093/oxfordjournals.jbchem.a123225.
8
Transmembrane signal transduction and osmoregulation in Escherichia coli: functional importance of the transmembrane regions of membrane-located protein kinase, EnvZ.大肠杆菌中的跨膜信号转导与渗透调节：膜定位蛋白激酶EnvZ跨膜区域的功能重要性
J Biochem. 1992 Jun;111(6):707-13. doi: 10.1093/oxfordjournals.jbchem.a123823.
9
The role of the G2 box, a conserved motif in the histidine kinase superfamily, in modulating the function of EnvZ.G2框作为组氨酸激酶超家族中的一个保守基序，在调节EnvZ功能中的作用。
Mol Microbiol. 2002 Aug;45(3):653-63. doi: 10.1046/j.1365-2958.2002.03061.x.
10
Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system.在大肠杆菌无细胞系统中，两个不需要EnvZ磷酸化的转录活性OmpR突变体。
J Bacteriol. 1992 Oct;174(20):6685-7. doi: 10.1128/jb.174.20.6685-6687.1992.
引用本文的文献
1
Robust and tunable signal processing in mammalian cells via engineered covalent modification cycles.通过工程化的共价修饰循环在哺乳动物细胞中实现稳健和可调的信号处理。
Nat Commun. 2022 Mar 31;13(1):1720. doi: 10.1038/s41467-022-29338-w.
2
New Insights into Multistep-Phosphorelay (MSP)/ Two-Component System (TCS) Regulation: Are Plants and Bacteria that Different?多步磷酸化（MSP）/双组分系统（TCS）调控的新见解：植物和细菌真的有那么不同吗？
Plants (Basel). 2019 Dec 11;8(12):590. doi: 10.3390/plants8120590.
3
Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices.通过在无序螺旋内关键组氨酸定位来设计渗透传感器。
Structure. 2019 Feb 5;27(2):302-314.e4. doi: 10.1016/j.str.2018.10.012. Epub 2018 Nov 29.
4
Sensory deprivation in Staphylococcus aureus.金黄色葡萄球菌中的感觉剥夺
Nat Commun. 2018 Feb 6;9(1):523. doi: 10.1038/s41467-018-02949-y.
5
Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action.激酶-调节因子复合物作用时的三维快照揭示的信号传导方向性调控
Elife. 2016 Dec 12;5:e21422. doi: 10.7554/eLife.21422.
6
Functional Dissection of the CroRS Two-Component System Required for Resistance to Cell Wall Stressors in Enterococcus faecalis.粪肠球菌中对细胞壁应激源产生抗性所需的CroRS双组分系统的功能剖析
J Bacteriol. 2016 Mar 31;198(8):1326-36. doi: 10.1128/JB.00995-15. Print 2016 Apr.
7
Specificity residues determine binding affinity for two-component signal transduction systems.特异性残基决定了二组分信号转导系统的结合亲和力。
mBio. 2013 Nov 5;4(6):e00420-13. doi: 10.1128/mBio.00420-13.
8
Genetic and biochemical dissection of a HisKA domain identifies residues required exclusively for kinase and phosphatase activities.对 HisKA 结构域的遗传和生化剖析确定了激酶和磷酸酶活性所必需的独特残基。
PLoS Genet. 2012;8(11):e1003084. doi: 10.1371/journal.pgen.1003084. Epub 2012 Nov 29.
9
The inner membrane histidine kinase EnvZ senses osmolality via helix-coil transitions in the cytoplasm.内膜组氨酸激酶EnvZ 通过细胞质中的螺旋-卷曲转变来感知渗透压。
EMBO J. 2012 May 30;31(11):2648-59. doi: 10.1038/emboj.2012.99. Epub 2012 Apr 27.
10
Negative control in two-component signal transduction by transmitter phosphatase activity.通过递质磷酸酶活性对双组分信号转导进行负调控。
Mol Microbiol. 2011 Oct;82(2):275-86. doi: 10.1111/j.1365-2958.2011.07829.x. Epub 2011 Sep 29.
本文引用的文献
1
Reverse phosphotransfer from OmpR to EnvZ in a kinase-/phosphatase+ mutant of EnvZ (EnvZ.N347D), a bifunctional signal transducer of Escherichia coli.在大肠杆菌双功能信号转导蛋白EnvZ的激酶功能缺失/磷酸酶功能增强突变体（EnvZ.N347D）中，OmpR向EnvZ的反向磷酸转移。
J Biol Chem. 1996 Jan 19;271(3):1424-9. doi: 10.1074/jbc.271.3.1424.
2
Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli.大肠杆菌中脂肪酸受体基因fadL的渗透调节
Mol Gen Genet. 1993 Sep;240(3):339-47. doi: 10.1007/BF00280384.
3
Requirement of both kinase and phosphatase activities of an Escherichia coli receptor (Taz1) for ligand-dependent signal transduction.大肠杆菌受体（Taz1）的激酶和磷酸酶活性对配体依赖性信号转导的要求。
J Mol Biol. 1993 May 20;231(2):335-42. doi: 10.1006/jmbi.1993.1286.
4
5
Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli.大肠杆菌渗透压感受器EnvZ磷酸化位点的鉴定。
J Biol Chem. 1994 Mar 25;269(12):8728-33.
6
7
Identification of a phosphorylation site and functional analysis of conserved aspartic acid residues of OmpR, a transcriptional activator for ompF and ompC in Escherichia coli.大肠杆菌中ompF和ompC的转录激活因子OmpR的磷酸化位点鉴定及保守天冬氨酸残基的功能分析。
Mol Microbiol. 1993 Dec;10(5):1037-47. doi: 10.1111/j.1365-2958.1993.tb00974.x.
8
Mutational analysis of the bacterial signal-transducing protein kinase/phosphatase nitrogen regulator II (NRII or NtrB).细菌信号转导蛋白激酶/磷酸酶氮调节蛋白II（NRII或NtrB）的突变分析
J Bacteriol. 1993 Nov;175(21):7016-23. doi: 10.1128/jb.175.21.7016-7023.1993.
9
Signal transduction by the EnvZ-OmpR phosphotransfer system in bacteria.
Res Microbiol. 1994 Jun-Aug;145(5-6):363-73. doi: 10.1016/0923-2508(94)90083-3.
10
The NarX and NarQ sensor-transmitter proteins of Escherichia coli each require two conserved histidines for nitrate-dependent signal transduction to NarL.大肠杆菌的NarX和NarQ传感-传递蛋白各自需要两个保守的组氨酸来进行依赖硝酸盐的信号转导至NarL。
J Bacteriol. 1995 May;177(9):2416-24. doi: 10.1128/jb.177.9.2416-2424.1995.
Zotero 插件