失活的质粒起始蛋白RepC/RepC*可能具有调控作用。
The inactivated plasmid inititator protein RepC/RepC* may have a regulatory role.
作者信息
Rasooly A, Rasooly R S
机构信息
Skirball Institute, New York University Medical Center, New York, USA.
出版信息
J Bacteriol. 1997 Feb;179(4):1420-2. doi: 10.1128/jb.179.4.1420-1422.1997.
Abstract
During replication of the plasmid pT181, the initiator protein RepC is modified by the addition of an oligodeoxynucleotide, giving rise to a new form, RepC*. Here we show that during in vitro replication, RepC* is radioactively labeled, suggesting that the source of the RepC* oligodeoxynucleotide is the newly synthesized pT181 DNA. The RepC/RepC* heterodimer retains its ability to bind the pT181 double-strand origin and, therefore, it may act as a competitive inhibitor of the RepC homodimer during replication.
摘要
在质粒pT181复制期间，起始蛋白RepC通过添加寡聚脱氧核苷酸进行修饰，产生一种新形式，即RepC*。在此我们表明，在体外复制过程中，RepC被放射性标记，这表明RepC寡聚脱氧核苷酸的来源是新合成的pT181 DNA。RepC/RepC*异二聚体保留了结合pT181双链起点的能力，因此，它可能在复制过程中作为RepC同二聚体的竞争性抑制剂发挥作用。
相似文献
1
The inactivated plasmid inititator protein RepC/RepC* may have a regulatory role.失活的质粒起始蛋白RepC/RepC*可能具有调控作用。
J Bacteriol. 1997 Feb;179(4):1420-2. doi: 10.1128/jb.179.4.1420-1422.1997.
2
Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer.
EMBO J. 1994 Nov 1;13(21):5245-51. doi: 10.1002/j.1460-2075.1994.tb06856.x.
3
In vitro inhibitory activity of RepC/C*, the inactivated form of the pT181 plasmid initiation protein, RepC.pT181质粒起始蛋白RepC的失活形式RepC/C*的体外抑制活性
J Bacteriol. 1997 Jan;179(1):141-7. doi: 10.1128/jb.179.1.141-147.1997.
4
An oligonucleotide inhibits oligomerization of a rolling circle initiator protein at the pT181 origin of replication.一种寡核苷酸在pT181复制起点抑制滚环引发蛋白的寡聚化。
J Biol Chem. 1998 Jun 26;273(26):16082-9. doi: 10.1074/jbc.273.26.16082.
5
Double-stranded origin nicking and replication initiation are coupled in the replication of a rolling circle plasmid, pT181.双链复制起点切口与复制起始在滚环质粒pT181的复制过程中相互关联。
FEMS Microbiol Lett. 1997 Jun 15;151(2):185-9. doi: 10.1111/j.1574-6968.1997.tb12568.x.
6
The PcrA3 mutant binds DNA and interacts with the RepC initiator protein of plasmid pT181 but is defective in its DNA helicase and unwinding activities.PcrA3突变体可结合DNA，并与质粒pT181的RepC起始蛋白相互作用，但其DNA解旋酶和解链活性存在缺陷。
Plasmid. 2005 Sep;54(2):104-13. doi: 10.1016/j.plasmid.2005.01.004. Epub 2005 Mar 4.
7
Specificity of RepC protein in plasmid pT181 DNA replication.RepC蛋白在质粒pT181 DNA复制中的特异性
J Biol Chem. 1990 Feb 25;265(6):3484-8.
8
The inactive pT181 initiator heterodimer, RepC/C, binds but fails to induce melting of the plasmid replication origin.无活性的pT181起始异二聚体RepC/C能够结合，但无法诱导质粒复制起点解链。
J Biol Chem. 1996 Dec 6;271(49):31086-91. doi: 10.1074/jbc.271.49.31086.
9
An 18-base-pair sequence is sufficient for termination of rolling-circle replication of plasmid pT181.一段18个碱基对的序列足以终止质粒pT181的滚环复制。
J Bacteriol. 1996 Sep;178(17):5222-8. doi: 10.1128/jb.178.17.5222-5228.1996.
10
Replication-specific inactivation of the pT181 plasmid initiator protein.pT181质粒起始蛋白的复制特异性失活。
Science. 1993 Nov 12;262(5136):1048-50. doi: 10.1126/science.8235621.
本文引用的文献
1
Replication-specific inactivation of the pT181 plasmid initiator protein.pT181质粒起始蛋白的复制特异性失活。
Science. 1993 Nov 12;262(5136):1048-50. doi: 10.1126/science.8235621.
2
Plasmids of the pT181 family show replication-specific initiator protein modification.pT181家族的质粒表现出复制特异性起始蛋白修饰。
J Bacteriol. 1994 Apr;176(8):2450-3. doi: 10.1128/jb.176.8.2450-2453.1994.
3
Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer.
EMBO J. 1994 Nov 1;13(21):5245-51. doi: 10.1002/j.1460-2075.1994.tb06856.x.
4
Active site of the replication protein of the rolling circle plasmid pC194.滚环质粒pC194复制蛋白的活性位点。
EMBO J. 1994 Sep 15;13(18):4412-20. doi: 10.1002/j.1460-2075.1994.tb06761.x.
5
The level of the pUB110 replication initiator protein is autoregulated, which provides an additional control for plasmid copy number.pUB110复制起始蛋白的水平受到自动调节，这为质粒拷贝数提供了额外的控制。
Nucleic Acids Res. 1995 Jun 11;23(11):1894-900. doi: 10.1093/nar/23.11.1894.
6
Replication of plasmid pT181 DNA in vitro: requirement for a plasmid-encoded product.质粒pT181 DNA的体外复制：对质粒编码产物的需求
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4902-6. doi: 10.1073/pnas.78.8.4902.
7
Control of pT181 replication II. Mutational analysis.pT181复制的控制II. 突变分析
EMBO J. 1984 Oct;3(10):2407-14. doi: 10.1002/j.1460-2075.1984.tb02147.x.
8
Regulated expression of a gene important for replication of plasmid F in E. coli.对质粒F在大肠杆菌中复制起重要作用的一个基因的调控表达。
EMBO J. 1984 Feb;3(2):257-62. doi: 10.1002/j.1460-2075.1984.tb01794.x.
9
Regulation of plasmid replication.质粒复制的调控
Microbiol Rev. 1984 Mar;48(1):1-23. doi: 10.1128/mr.48.1.1-23.1984.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
Zotero 插件