对质粒F在大肠杆菌中复制起重要作用的一个基因的调控表达。
Regulated expression of a gene important for replication of plasmid F in E. coli.
作者信息
Søgaard-Andersen L, Rokeach L A, Molin S
出版信息
EMBO J. 1984 Feb;3(2):257-62. doi: 10.1002/j.1460-2075.1984.tb01794.x.
Abstract
Fusions between the gene encoding the E protein of the IncFI plasmid F and the lac genes were constructed. Analysis of the expression of beta-galactosidase from these fusions shows that the promoter for the E protein gene is located between the incB region and the structural gene for the E protein. Near this promoter is a regulatory site on which a negative control element acts. Most likely the E protein itself acts as a repressor of E gene expression and thus autoregulates its own expression. No other gene products seem to affect the expression of the E protein gene.
摘要
构建了编码IncFI质粒F的E蛋白的基因与lac基因之间的融合体。对这些融合体中β-半乳糖苷酶表达的分析表明，E蛋白基因的启动子位于incB区域和E蛋白的结构基因之间。在该启动子附近有一个调控位点，一个负调控元件作用于其上。很可能E蛋白本身作为E基因表达的阻遏物，从而对其自身表达进行自动调节。似乎没有其他基因产物影响E蛋白基因的表达。
相似文献
1
Regulated expression of a gene important for replication of plasmid F in E. coli.对质粒F在大肠杆菌中复制起重要作用的一个基因的调控表达。
EMBO J. 1984 Feb;3(2):257-62. doi: 10.1002/j.1460-2075.1984.tb01794.x.
2
Suppression of replication-deficient mutants of IncFII plasmid NR1 can occur by two different mechanisms that increase expression of the repA1 gene.FII型质粒NR1复制缺陷型突变体的抑制可通过两种不同机制发生，这两种机制均可增加repA1基因的表达。
J Bacteriol. 1993 May;175(10):3161-73. doi: 10.1128/jb.175.10.3161-3173.1993.
3
In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101.质粒pSC101的RepA复制蛋白拷贝数突变的体内和体外研究
J Bacteriol. 1993 Jul;175(13):4165-75. doi: 10.1128/jb.175.13.4165-4175.1993.
4
Mechanism of autonomous control of the Escherichia coli F plasmid: different complexes of the initiator/repressor protein are bound to its operator and to an F plasmid replication origin.大肠杆菌F质粒自主控制机制：起始/阻遏蛋白的不同复合物与它的操纵基因及F质粒复制起点结合。
Nucleic Acids Res. 1986 Jul 25;14(14):5693-711. doi: 10.1093/nar/14.14.5693.
5
A versatile low-copy-number cloning vector derived from plasmid F.一种源自质粒F的多功能低拷贝数克隆载体。
Gene. 1995 Oct 16;164(1):55-8. doi: 10.1016/0378-1119(95)00419-7.
6
The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator.氧化还原调节的SoxR蛋白在单个DNA位点发挥作用，既是一种阻遏物，也是一种变构激活剂。
EMBO J. 1998 May 1;17(9):2629-36. doi: 10.1093/emboj/17.9.2629.
7
Regulatory mechanisms in expression of the traY-I operon of sex factor plasmid R100: involvement of traJ and traY gene products.性因子质粒R100的traY-I操纵子表达中的调控机制：traJ和traY基因产物的作用。
Genes Cells. 1998 Jun;3(6):331-45. doi: 10.1046/j.1365-2443.1998.00194.x.
8
Two functions of the E protein are key elements in the plasmid F replication control system.E蛋白的两种功能是质粒F复制控制系统中的关键要素。
J Bacteriol. 1985 Dec;164(3):1262-70. doi: 10.1128/jb.164.3.1262-1270.1985.
9
Replication and incompatibility of F and plasmids in the IncFI Group.F质粒及IncFI组中质粒的复制与不相容性
Plasmid. 1981 Jan;5(1):100-26. doi: 10.1016/0147-619x(81)90079-2.
10
Multiple mechanisms contribute to osmotic inducibility of proU operon expression in Escherichia coli: demonstration of two osmoresponsive promoters and of a negative regulatory element within the first structural gene.多种机制促成了大肠杆菌中proU操纵子表达的渗透诱导性：两个渗透反应启动子及第一个结构基因内一个负调控元件的证明。
J Bacteriol. 1991 Dec;173(23):7481-90. doi: 10.1128/jb.173.23.7481-7490.1991.
引用本文的文献
1
Discovery of small-molecule inhibitors of multidrug-resistance plasmid maintenance using a high-throughput screening approach.采用高通量筛选方法发现多药耐药质粒维持的小分子抑制剂。
Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29839-29850. doi: 10.1073/pnas.2005948117. Epub 2020 Nov 9.
2
The inactivated plasmid inititator protein RepC/RepC* may have a regulatory role.失活的质粒起始蛋白RepC/RepC*可能具有调控作用。
J Bacteriol. 1997 Feb;179(4):1420-2. doi: 10.1128/jb.179.4.1420-1422.1997.
3
Measurement of gene expression by translational coupling: effect of copy mutations on pT181 initiator synthesis.通过翻译偶联测量基因表达：拷贝突变对pT181起始子合成的影响。
EMBO J. 1993 Sep;12(9):3659-67. doi: 10.1002/j.1460-2075.1993.tb06040.x.
4
Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).微小F质粒的复制起始蛋白RepE：单体（起始蛋白）与二聚体（自体阻遏物）之间的功能分化
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3839-43. doi: 10.1073/pnas.91.9.3839.
5
Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer.
EMBO J. 1994 Nov 1;13(21):5245-51. doi: 10.1002/j.1460-2075.1994.tb06856.x.
6
Location by DNA sequence analysis of cop mutations affecting the number of plasmid copies of prophage P1.通过DNA序列分析确定影响原噬菌体P1质粒拷贝数的cop突变的位置。
Mol Gen Genet. 1984;194(3):513-6. doi: 10.1007/BF00425567.
7
Regulation of the F-factor pif operon: pifO, a site required in cis for autoregulation, titrates the pifC product in trans.F 因子 pif 操纵子的调控：pifO，顺式作用下自身调控所需的位点，反式作用下滴定 pifC 产物。
J Bacteriol. 1984 Oct;160(1):192-8. doi: 10.1128/jb.160.1.192-198.1984.
8
korA function of promiscuous plasmid RK2: an autorepressor that inhibits expression of host-lethal gene kilA and replication gene trfA.滥交质粒RK2的KorA功能：一种自抑制因子，可抑制宿主致死基因kilA和复制基因trfA的表达。
Proc Natl Acad Sci U S A. 1985 Nov;82(21):7374-8. doi: 10.1073/pnas.82.21.7374.
9
P1 plasmid replication: multiple functions of RepA protein at the origin.P1质粒复制：RepA蛋白在复制起点的多种功能。
Proc Natl Acad Sci U S A. 1985 May;82(9):2588-92. doi: 10.1073/pnas.82.9.2588.
10
Transient suppression of F-plasmid incompatibility in a strain of Escherichia coli.大肠杆菌菌株中F质粒不相容性的瞬时抑制
Mol Gen Genet. 1986 Sep;204(3):463-8. doi: 10.1007/BF00331025.
本文引用的文献
1
Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.溶源性研究。I. 溶源性大肠杆菌释放噬菌体的方式。
J Bacteriol. 1951 Sep;62(3):293-300. doi: 10.1128/jb.62.3.293-300.1951.
2
Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule.由小RNA分子介导的质粒R1的repA基因表达的转录后调控
EMBO J. 1983;2(1):93-8. doi: 10.1002/j.1460-2075.1983.tb01387.x.
3
Vertical dye-buoyant density gradients for rapid analysis and preparation of plasmid DNA.用于快速分析和制备质粒DNA的垂直染料浮力密度梯度
Anal Biochem. 1981 Nov 15;118(1):191-3. doi: 10.1016/0003-2697(81)90177-9.
4
Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.通过对切割后的miniF片段进行克隆分析来检测和定位六种由miniF编码的蛋白质。
Mol Gen Genet. 1982;186(2):193-203. doi: 10.1007/BF00331850.
5
Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication.质粒R1的复制控制功能可作为复制所需基因表达的抑制剂。
Mol Gen Genet. 1981;184(1):56-61. doi: 10.1007/BF00271195.
6
Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.在微小F质粒复制和不相容性所必需的区域中的九个独特重复序列。
Gene. 1981 Nov;15(2-3):257-71. doi: 10.1016/0378-1119(81)90135-9.
7
Direct repeats of the F plasmid incC region express F incompatibility.F质粒incC区域的直接重复序列表达F不相容性。
Cell. 1981 Jun;24(3):687-94. doi: 10.1016/0092-8674(81)90095-7.
8
Plasmid mini-F encoded proteins.质粒微小F编码蛋白。
Mol Gen Genet. 1980;180(1):205-11. doi: 10.1007/BF00267371.
9
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.通过在大肠杆菌中进行DNA融合和克隆分析基因控制信号。
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
10
IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.IncD是F因子中的一个基因座，负责与IncFI组的几种质粒不亲和。
Mol Gen Genet. 1982;188(2):345-52. doi: 10.1007/BF00332699.
Zotero 插件